Novel H2-oxidizing [NiFeSe]hydrogenase from Desulfovibrio vulgaris Miyazaki F

Kyoshiro Nonaka, Nga T. Nguyen, Ki Suk Yoon, Seiji Ogo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

[NiFeSe]hydrogenases are promising biocatalysts in H2-based technology due to their high catalytic activity and O2-stability. Here, we report purification and characterization of a new membrane-associated [NiFeSe]hydrogenase from Desulfovibrio vulgaris Miyazaki F ([NiFeSe]DvMF). The [NiFeSe]DvMF was composed of two subunits, corresponding to a large subunit of 58.3 kDa and a small subunit of 29.3 kDa determined by SDS-PAGE. Unlike conventional [NiFeSe]hydrogenases having catalytic bias toward H2-production, the [NiFeSe]DvMF showed 11-fold higher specific activity of H2-oxidation (2444 U/mg) than that of H2-production (217 U/mg). At the optimal reaction temperature of the enzyme (65°C), the specific activity of H2-oxidation could reach up to 21,553 U/mg. Amperometric assays of the [NiFeSe]DvMF clearly indicated that the enzyme had a remarkable O2-stability. According to the amino acid sequence alignment, the conserved cysteine residue at position 281 in medial cluster of other [NiFeSe]hydrogenases was specifically replaced by a serine residue (Ser281) in the [NiFeSe]DvMF. These results indicate that the [NiFeSe]DvMF can play as a new H2-oxidizing and O2-stable biocatalyst, along with providing helpful insights into the structure-function relationship of [NiFeSe]hydrogenases.

Original languageEnglish
Pages (from-to)366-371
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume115
Issue number4
DOIs
Publication statusPublished - Apr 1 2013

Fingerprint

Desulfovibrio vulgaris
Hydrogenase
Biocatalysts
Enzymes
Oxidation
Purification
Amino acids
Assays
Catalyst activity
Thermodynamic properties
Membranes
Sequence Alignment
Serine
Cysteine
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Temperature
nickel-iron-selenium hydrogenase
Technology
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Novel H2-oxidizing [NiFeSe]hydrogenase from Desulfovibrio vulgaris Miyazaki F. / Nonaka, Kyoshiro; Nguyen, Nga T.; Yoon, Ki Suk; Ogo, Seiji.

In: Journal of Bioscience and Bioengineering, Vol. 115, No. 4, 01.04.2013, p. 366-371.

Research output: Contribution to journalArticle

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