Novel inhibitors of phenylalanine ammonia-lyase: 5-Aryl-1,3,4-oxathiazol-2- ones

Naotaka Yamada, Soonsung Hong, Eiichi Kuwano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A series of 5-aryl-1,3,4-oxathiazol-2-ones was synthesized and tested for inhibition against phenylalanine ammonia-lyase (PAL) derived from the yeast Rhodotorula glutinis. All compounds inhibited PAL at 100 μM. Of the compounds tested, 5-(4-nitrophenyl)-1,3,4-oxathiazol-2-one (19) was the most effective, showing IC 50 value of 2.5 μM, which was 2 times stronger than that of N-(aminooxy) acetyl-2,5-dichloroaniline (Z302), a known PAL inhibitor. Compound 19 behaved as a noncompetitive inhibitor of PAL. When potato tuber disks were treated with 19 in the presence of laminarin, a β-1,3- glucooligosaccharide elicitor, the amount of trans-cinnamic acid diminished after 48 hr, being consistent with an effective inhibition of PAL. Most of the compounds inhibited the growth of lettuce seedlings at 100-500 μM. However, no correlation was observed between the ability of 5-aryl-1,3,4-oxathiazol-2- ones to inhibit PAL derived from R. glutinis and growth inhibitory activity against lettuce seedlings.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume51
Issue number2
Publication statusPublished - Oct 1 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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