Novel Leu-Lys-specific peptidase (leulysin) produced by gel-entrapped yeast cells

Tsutomu Okada, Kenji Sonomoto, Atsuo Tanaka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Saccharomyces cerevisiae cells entrapped with a neutral hydrophilic photo-crosslinkable resin prepolymers specifically excreted into a cultured medium a new type of a peptidase, which cleaved Leu-Lys bond of α-mating factor. The enzyme was purified by membrane filtration followed by ion exchange chromatography and gel filtration chromatography. The purified enzyme showed a strict substrate specificity on internal Leu-Lys bond. Leu-Lys bond near terminus of a molecule and Leu-X and X-Lys bonds examined so far were not hydrolyzed by the enzyme.

Original languageEnglish
Pages (from-to)316-322
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume145
Issue number1
DOIs
Publication statusPublished - May 29 1987

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Yeast
Yeasts
Gels
Cells
Mating Factor
Chromatography
Enzymes
Ion Exchange Chromatography
Substrate Specificity
Gel Chromatography
Saccharomyces cerevisiae
Ion exchange
Peptide Hydrolases
Resins
Membranes
Molecules
Substrates
leulysin
leucyl-lysine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Novel Leu-Lys-specific peptidase (leulysin) produced by gel-entrapped yeast cells. / Okada, Tsutomu; Sonomoto, Kenji; Tanaka, Atsuo.

In: Biochemical and Biophysical Research Communications, Vol. 145, No. 1, 29.05.1987, p. 316-322.

Research output: Contribution to journalArticle

Okada, Tsutomu ; Sonomoto, Kenji ; Tanaka, Atsuo. / Novel Leu-Lys-specific peptidase (leulysin) produced by gel-entrapped yeast cells. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 145, No. 1. pp. 316-322.
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