Novel mouse xenograft models reveal a critical role of CD4 + T cells in the proliferation of ebv-infected T and NK cells

Ken Ichi Imadome, Misako Yajima, Ayako Arai, Atsuko Nakazawa, Fuyuko Kawano, Sayumi Ichikawa, Norio Shimizu, Naoki Yamamoto, Tomohiro Morio, Shouichi Ohga, Hiroyuki Nakamura, Mamoru Ito, Osamu Miura, Jun Komano, Shigeyoshi Fujiwara

Research output: Contribution to journalArticle

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Abstract

Epstein-Barr virus (EBV), a ubiquitous B-lymphotropic herpesvirus, ectopically infects T or NK cells to cause severe diseases of unknown pathogenesis, including chronic active EBV infection (CAEBV) and EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH). We developed xenograft models of CAEBV and EBV-HLH by transplanting patients' PBMC to immunodeficient mice of the NOD/Shi-scid/IL-2Rγ null strain. In these models, EBV-infected T, NK, or B cells proliferated systemically and reproduced histological characteristics of the two diseases. Analysis of the TCR repertoire expression revealed that identical predominant EBV-infected T-cell clones proliferated in patients and corresponding mice transplanted with their PBMC. Expression of the EBV nuclear antigen 1 (EBNA1), the latent membrane protein 1 (LMP1), and LMP2, but not EBNA2, in the engrafted cells is consistent with the latency II program of EBV gene expression known in CAEBV. High levels of human cytokines, including IL-8, IFN-γ, and RANTES, were detected in the peripheral blood of the model mice, mirroring hypercytokinemia characteristic to both CAEBV and EBV-HLH. Transplantation of individual immunophenotypic subsets isolated from patients' PBMC as well as that of various combinations of these subsets revealed a critical role of CD4 + T cells in the engraftment of EBV-infected T and NK cells. In accordance with this finding, in vivo depletion of CD4 + T cells by the administration of the OKT4 antibody following transplantation of PBMC prevented the engraftment of EBV-infected T and NK cells. This is the first report of animal models of CAEBV and EBV-HLH that are expected to be useful tools in the development of novel therapeutic strategies for the treatment of the diseases.

Original languageEnglish
Article numbere1002326
JournalPLoS pathogens
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 1 2011

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Human Herpesvirus 4
Heterografts
Natural Killer Cells
Cell Proliferation
T-Lymphocytes
Epstein-Barr Virus Infections
Transplantation
Epstein-Barr Virus Nuclear Antigens
Hemophagocytic Lymphohistiocytosis
Cercopithecine Herpesvirus 1
Chemokine CCL5
Inbred NOD Mouse
Interleukin-8
Membrane Proteins
B-Lymphocytes
Animal Models
Clone Cells
Cytokines
Gene Expression
Antibodies

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Imadome, K. I., Yajima, M., Arai, A., Nakazawa, A., Kawano, F., Ichikawa, S., ... Fujiwara, S. (2011). Novel mouse xenograft models reveal a critical role of CD4 + T cells in the proliferation of ebv-infected T and NK cells. PLoS pathogens, 7(10), [e1002326]. https://doi.org/10.1371/journal.ppat.1002326

Novel mouse xenograft models reveal a critical role of CD4 + T cells in the proliferation of ebv-infected T and NK cells. / Imadome, Ken Ichi; Yajima, Misako; Arai, Ayako; Nakazawa, Atsuko; Kawano, Fuyuko; Ichikawa, Sayumi; Shimizu, Norio; Yamamoto, Naoki; Morio, Tomohiro; Ohga, Shouichi; Nakamura, Hiroyuki; Ito, Mamoru; Miura, Osamu; Komano, Jun; Fujiwara, Shigeyoshi.

In: PLoS pathogens, Vol. 7, No. 10, e1002326, 01.10.2011.

Research output: Contribution to journalArticle

Imadome, KI, Yajima, M, Arai, A, Nakazawa, A, Kawano, F, Ichikawa, S, Shimizu, N, Yamamoto, N, Morio, T, Ohga, S, Nakamura, H, Ito, M, Miura, O, Komano, J & Fujiwara, S 2011, 'Novel mouse xenograft models reveal a critical role of CD4 + T cells in the proliferation of ebv-infected T and NK cells', PLoS pathogens, vol. 7, no. 10, e1002326. https://doi.org/10.1371/journal.ppat.1002326
Imadome, Ken Ichi ; Yajima, Misako ; Arai, Ayako ; Nakazawa, Atsuko ; Kawano, Fuyuko ; Ichikawa, Sayumi ; Shimizu, Norio ; Yamamoto, Naoki ; Morio, Tomohiro ; Ohga, Shouichi ; Nakamura, Hiroyuki ; Ito, Mamoru ; Miura, Osamu ; Komano, Jun ; Fujiwara, Shigeyoshi. / Novel mouse xenograft models reveal a critical role of CD4 + T cells in the proliferation of ebv-infected T and NK cells. In: PLoS pathogens. 2011 ; Vol. 7, No. 10.
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