Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis

Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to conferencePaper

Abstract

For protein analysis, polymeric microchip was extremely incompatible with protein samples. To overcome the incompatibility, we developed a novel physically adsorbed polymer coating, which was a new approach and different from conventional coating methods, on PMMA microchip using cellulose derivatives as the coating reagents. The coating method remarkably progressed analytical performance and we could successfully separate 5 proteins dependent of their own sizes with high reproducibility (RSD; within 3 %, n=12) on coated PMMA microchip with 12 channels array.

Original languageEnglish
Pages401-403
Number of pages3
Publication statusPublished - Jan 1 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

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Polymethyl Methacrylate
Polymers
Throughput
Proteins
Coatings
Cellulose derivatives

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Okada, H., Kaji, N., Tokeshi, M., & Baba, Y. (2006). Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis. 401-403. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis. / Okada, Hiroki; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

2006. 401-403 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conferencePaper

Okada, H, Kaji, N, Tokeshi, M & Baba, Y 2006, 'Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis', Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06 - 11/9/06 pp. 401-403.
Okada H, Kaji N, Tokeshi M, Baba Y. Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis. 2006. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.
Okada, Hiroki ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.3 p.
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