Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis

Hiroki Okada; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis

Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to conference › Paper › peer-review

Abstract

For protein analysis, polymeric microchip was extremely incompatible with protein samples. To overcome the incompatibility, we developed a novel physically adsorbed polymer coating, which was a new approach and different from conventional coating methods, on PMMA microchip using cellulose derivatives as the coating reagents. The coating method remarkably progressed analytical performance and we could successfully separate 5 proteins dependent of their own sizes with high reproducibility (RSD; within 3 %, n=12) on coated PMMA microchip with 12 channels array.

Original language	English
Pages	401-403
Number of pages	3
Publication status	Published - Jan 1 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

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Novel physically adsorbed polymer coating on PMMA microchip with multi channel array for high-throughput protein analysis. / Okada, Hiroki; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

2006. 401-403 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

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AU - Baba, Yoshinobu

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AB - For protein analysis, polymeric microchip was extremely incompatible with protein samples. To overcome the incompatibility, we developed a novel physically adsorbed polymer coating, which was a new approach and different from conventional coating methods, on PMMA microchip using cellulose derivatives as the coating reagents. The coating method remarkably progressed analytical performance and we could successfully separate 5 proteins dependent of their own sizes with high reproducibility (RSD; within 3 %, n=12) on coated PMMA microchip with 12 channels array.

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