Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells

Hiroshi Yukawa, Shingo Nakagawa, Yasuma Yoshizumi, Masaki Watanabe, Hiroaki Saito, Yoshitaka Miyamoto, Hirofumi Noguchi, Koichi Oishi, Kenji Ono, Makoto Sawada, Ichiro Kato, Daisuke Onoshima, Momoko Obayashi, Yumi Hayashi, Noritada Kaji, Tetsuya Ishikawa, Shuji Hayashi, Yoshinobu Baba

Research output: Contribution to journalArticle

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Abstract

Stem cell transplantation has been expected to have various applications for regenerative medicine. However, in order to detect and trace the transplanted stem cells in the body, non-invasive and widely clinically available cell imaging technologies are required. In this paper, we focused on magnetic resonance (MR) imaging technology, and investigated whether the trimethylamino dextran-coated magnetic iron oxide nanoparticle -03 (TMADM-03), which was newly developed by our group, could be used for labeling adipose tissue-derived stem cells (ASCs) as a contrast agent. No cytotoxicity was observed in ASCs transduced with less than 100 μg-Fe/mL of TMADM-03 after a one hour transduction time. The transduction efficiency of TMADM-03 into ASCs was about four-fold more efficient than that of the alkali-treated dextrancoated magnetic iron oxide nanoparticle (ATDM), which is a major component of commercially available contrast agents such as ferucarbotran (Resovist), and the level of labeling was maintained for at least two weeks. In addition, the differentiation ability of ASCs labeled with TMADM-03 and their ability to produce cytokines such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2), were confirmed to be maintained. The ASCs labeled with TMADM-03 were transplanted into the left kidney capsule of a mouse. The labeled ASCs could be imaged with good contrast using a 1T MR imaging system. These data suggest that TMADM-03 can therefore be utilized as a contrast agent for the MR imaging of stem cells.

Original languageEnglish
Article numbere110142
JournalPloS one
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 3 2014
Externally publishedYes

Fingerprint

nanoparticles
Stem cells
Nanoparticles
Labeling
adipose tissue
stem cells
iron oxides
Adipose Tissue
Stem Cells
Dextrans
image analysis
dextran
Tissue
Imaging techniques
Magnetic resonance
Contrast Media
magnetic resonance imaging
Magnetic Resonance Imaging
Technology
hepatocyte growth factor

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Yukawa, H., Nakagawa, S., Yoshizumi, Y., Watanabe, M., Saito, H., Miyamoto, Y., ... Baba, Y. (2014). Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells. PloS one, 9(11), [e110142]. https://doi.org/10.1371/journal.pone.0110142

Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells. / Yukawa, Hiroshi; Nakagawa, Shingo; Yoshizumi, Yasuma; Watanabe, Masaki; Saito, Hiroaki; Miyamoto, Yoshitaka; Noguchi, Hirofumi; Oishi, Koichi; Ono, Kenji; Sawada, Makoto; Kato, Ichiro; Onoshima, Daisuke; Obayashi, Momoko; Hayashi, Yumi; Kaji, Noritada; Ishikawa, Tetsuya; Hayashi, Shuji; Baba, Yoshinobu.

In: PloS one, Vol. 9, No. 11, e110142, 03.11.2014.

Research output: Contribution to journalArticle

Yukawa, H, Nakagawa, S, Yoshizumi, Y, Watanabe, M, Saito, H, Miyamoto, Y, Noguchi, H, Oishi, K, Ono, K, Sawada, M, Kato, I, Onoshima, D, Obayashi, M, Hayashi, Y, Kaji, N, Ishikawa, T, Hayashi, S & Baba, Y 2014, 'Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells', PloS one, vol. 9, no. 11, e110142. https://doi.org/10.1371/journal.pone.0110142
Yukawa H, Nakagawa S, Yoshizumi Y, Watanabe M, Saito H, Miyamoto Y et al. Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells. PloS one. 2014 Nov 3;9(11). e110142. https://doi.org/10.1371/journal.pone.0110142
Yukawa, Hiroshi ; Nakagawa, Shingo ; Yoshizumi, Yasuma ; Watanabe, Masaki ; Saito, Hiroaki ; Miyamoto, Yoshitaka ; Noguchi, Hirofumi ; Oishi, Koichi ; Ono, Kenji ; Sawada, Makoto ; Kato, Ichiro ; Onoshima, Daisuke ; Obayashi, Momoko ; Hayashi, Yumi ; Kaji, Noritada ; Ishikawa, Tetsuya ; Hayashi, Shuji ; Baba, Yoshinobu. / Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells. In: PloS one. 2014 ; Vol. 9, No. 11.
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