Novel preparation method for surfactant-lipase complexes utilizing water in oil emulsions

Shin Ya Okazaki; Noriho Kamiya; Kojiro Abe; Masahiro Goto; Fumiyuki Nakashio

doi:10.1002/(SICI)1097-0290(19970720)55:2<455::AID-BIT23>3.0.CO;2-E

Novel preparation method for surfactant-lipase complexes utilizing water in oil emulsions

Shin Ya Okazaki, Noriho Kamiya, Kojiro Abe, Masahiro Goto, Fumiyuki Nakashio

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

A novel preparation method for surfactant-lipase complexes has been developed utilizing water in oil emulsions. In order to optimize the preparation conditions, we have investigated the effects of several operational parameters on the enzymatic activity of the surfactant-lipase complexes in organic media. When a nonionic surfactant was employed under optimal preparation conditions [alkaline pH 8-10, organic/aqueous = 90/10 (v/v), concentration of surfactant, 10 mM], the surfactant-lipase complex efficiently catalyzed the esterification of benzyl alcohol with lauric acid in organic media. The esterification rate of the surfactant-lipase complex was increased over 16-fold relative to the native powder lipase. Furthermore, the lipase complex showed high storage stability.

Original language	English
Pages (from-to)	455-460
Number of pages	6
Journal	Biotechnology and Bioengineering
Volume	55
Issue number	2
DOIs	https://doi.org/10.1002/(SICI)1097-0290(19970720)55:2<455::AID-BIT23>3.0.CO;2-E
Publication status	Published - Jul 20 1997

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/(SICI)1097-0290(19970720)55:2<455::AID-BIT23>3.0.CO;2-E

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abstract = "A novel preparation method for surfactant-lipase complexes has been developed utilizing water in oil emulsions. In order to optimize the preparation conditions, we have investigated the effects of several operational parameters on the enzymatic activity of the surfactant-lipase complexes in organic media. When a nonionic surfactant was employed under optimal preparation conditions [alkaline pH 8-10, organic/aqueous = 90/10 (v/v), concentration of surfactant, 10 mM], the surfactant-lipase complex efficiently catalyzed the esterification of benzyl alcohol with lauric acid in organic media. The esterification rate of the surfactant-lipase complex was increased over 16-fold relative to the native powder lipase. Furthermore, the lipase complex showed high storage stability.",

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AU - Okazaki, Shin Ya

AU - Kamiya, Noriho

AU - Abe, Kojiro

AU - Goto, Masahiro

AU - Nakashio, Fumiyuki

PY - 1997/7/20

Y1 - 1997/7/20

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AB - A novel preparation method for surfactant-lipase complexes has been developed utilizing water in oil emulsions. In order to optimize the preparation conditions, we have investigated the effects of several operational parameters on the enzymatic activity of the surfactant-lipase complexes in organic media. When a nonionic surfactant was employed under optimal preparation conditions [alkaline pH 8-10, organic/aqueous = 90/10 (v/v), concentration of surfactant, 10 mM], the surfactant-lipase complex efficiently catalyzed the esterification of benzyl alcohol with lauric acid in organic media. The esterification rate of the surfactant-lipase complex was increased over 16-fold relative to the native powder lipase. Furthermore, the lipase complex showed high storage stability.

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Novel preparation method for surfactant-lipase complexes utilizing water in oil emulsions

Abstract

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