Novel protein refolding by reversed micelles

M. Goto, T. Fujita, M. Sakono, S. Furusaki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A novel protein refolding technique utilizing reversed micelles has been developed. Denatured RNase A solubilized in the reversed micellar solution was reactivated by addition of glutathione entrapped in another reversed micellar solution. This novel refolding method facilitates a high renaturation yield at high protein concentration compared to achievable results in dilution method. Novel refolding technique by reversed micelles makes it possible to activate proteins from insoluble aggregates by direct dissolution into the reversed micellar solutions.

Original languageEnglish
Title of host publicationProgress in Biotechnology
PublisherElsevier
Pages113-118
Number of pages6
EditionC
DOIs
Publication statusPublished - 2000

Publication series

NameProgress in Biotechnology
NumberC
Volume16
ISSN (Print)0921-0423

All Science Journal Classification (ASJC) codes

  • Biotechnology

Fingerprint Dive into the research topics of 'Novel protein refolding by reversed micelles'. Together they form a unique fingerprint.

Cite this