Novel regulation of MHC class II function in B cells

Yohei Matsuki, Mari Ohmura-Hoshino, Eiji Goto, Masami Aoki, Mari Mito-Yoshida, Mika Uematsu, Takanori Hasegawa, Haruhiko Koseki, Osamu Ohara, Manabu Nakayama, Kiminori Toyooka, Ken Matsuoka, Hak Hotta, Akitsugu Yamamoto, Satoshi Ishido

Research output: Contribution to journalArticle

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Abstract

The presence of post-translational regulation of MHC class II (MHC II) under physiological conditions has been demonstrated recently in dendritic cells (DCs) that potently function as antigen-presenting cells (APCs). Here, we report that MARCH-I, an E3 ubiquitin ligase, plays a pivotal role in the post-translational regulation of MHC II in B cells. MARCH-I expression was particularly high in B cells, and the forced expression of MARCH-I induced the ubiquitination of MHC II. In B cells from MARCH-I-deficient mice (MARCH-I KO), the half-life of surface MHC II was prolonged and the ubiquitinated form of MHC II completely disappeared. In addition, MARCH-I-deficient B cells highly expressed exogenous antigen-loaded MHC II on their surface and showed high ability to present exogenous antigens. These results suggest that the function of MHC II in B cells is regulated through ubiquitination by MARCH-I.

Original languageEnglish
Pages (from-to)846-854
Number of pages9
JournalEMBO Journal
Volume26
Issue number3
DOIs
Publication statusPublished - Feb 7 2007

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B-Lymphocytes
Cells
Ubiquitination
Antigens
Ubiquitin-Protein Ligases
Antigen-Presenting Cells
Dendritic Cells
Half-Life

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Matsuki, Y., Ohmura-Hoshino, M., Goto, E., Aoki, M., Mito-Yoshida, M., Uematsu, M., ... Ishido, S. (2007). Novel regulation of MHC class II function in B cells. EMBO Journal, 26(3), 846-854. https://doi.org/10.1038/sj.emboj.7601556

Novel regulation of MHC class II function in B cells. / Matsuki, Yohei; Ohmura-Hoshino, Mari; Goto, Eiji; Aoki, Masami; Mito-Yoshida, Mari; Uematsu, Mika; Hasegawa, Takanori; Koseki, Haruhiko; Ohara, Osamu; Nakayama, Manabu; Toyooka, Kiminori; Matsuoka, Ken; Hotta, Hak; Yamamoto, Akitsugu; Ishido, Satoshi.

In: EMBO Journal, Vol. 26, No. 3, 07.02.2007, p. 846-854.

Research output: Contribution to journalArticle

Matsuki, Y, Ohmura-Hoshino, M, Goto, E, Aoki, M, Mito-Yoshida, M, Uematsu, M, Hasegawa, T, Koseki, H, Ohara, O, Nakayama, M, Toyooka, K, Matsuoka, K, Hotta, H, Yamamoto, A & Ishido, S 2007, 'Novel regulation of MHC class II function in B cells', EMBO Journal, vol. 26, no. 3, pp. 846-854. https://doi.org/10.1038/sj.emboj.7601556
Matsuki Y, Ohmura-Hoshino M, Goto E, Aoki M, Mito-Yoshida M, Uematsu M et al. Novel regulation of MHC class II function in B cells. EMBO Journal. 2007 Feb 7;26(3):846-854. https://doi.org/10.1038/sj.emboj.7601556
Matsuki, Yohei ; Ohmura-Hoshino, Mari ; Goto, Eiji ; Aoki, Masami ; Mito-Yoshida, Mari ; Uematsu, Mika ; Hasegawa, Takanori ; Koseki, Haruhiko ; Ohara, Osamu ; Nakayama, Manabu ; Toyooka, Kiminori ; Matsuoka, Ken ; Hotta, Hak ; Yamamoto, Akitsugu ; Ishido, Satoshi. / Novel regulation of MHC class II function in B cells. In: EMBO Journal. 2007 ; Vol. 26, No. 3. pp. 846-854.
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