Novel surface plasmon resonance (SPR) immunosensor based on monomolecular layer of physically-adsorbed ovalbumin conjugate for detection of 2,4-dichlorophenoxyacetic acid and atomic force microscopy study

K. Vengatajalabathy Gobi, Sook Jin Kim, Hiroyuki Tanaka, Yukihiro Shoyama, Norio Miura

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A rapid and simple optical immunosensor for detection of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has been developed based on SPR technology. Functional sensing surface of the immunosensor is created by immobilizing an ovalbumin conjugate of 2,4-D (2,4-D-OVA) by simple physical adsorption on an SPR thin-film gold chip. It has been established that the Au surface of the sensor chip was completely covered by 2,4-D-OVA up to a monomolecular layer and that the 2,4-D-OVA immobilized sensor chip was highly resistive to non-specific binding of proteins. Selective binding of a monoclonal antibody against 2,4-D (2,4-D-Ab) is followed by an increase in SPR angle. The antibody complexed on the sensor surface could be removed simply by the flow of an acidic buffer (glycine.HCl; 0.2 M, pH 2.0) for less than 1 min, facilitating repeated use of a same sensor chip. A competitive immunosensing method has been applied for the detection of 2,4-D, in which binding of the antibody onto the sensor surface in the presence and absence of 2,4-D is investigated. When 2,4-D is present in sample solution, a competition is set off between 2,4-D in solution and 2,4-D-OVA conjugate on sensor chip for binding to 2,4-D-Ab. A lowest detection limit of 0.1 ng/ml 2,4-D is established. Calibration curve of this analytical system covers a wide concentration range of 0.1-300 ng/ml 2,4-D. One assay could be completed in 18 min (binding, 15 min; acidic eluent and followed carrier buffer, 3 min). Enzyme-linked immunosorbent assay (ELISA) measurements for the detection of 2,4-D using analogous antigen-coat format showed a detection limit of 500 ng/ml. The high sensitivity of the configured SPR immunosensor system and the differences between the performances of SPR and ELISA are discussed. Cross-reactivity of the SPR sensor against a few compounds structurally and environmentally relevant to 2,4-D is examined. The fabricated SPR sensor is found to be highly resistant to interference with a maximum cross-reactivity of only 4% for 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).

Original languageEnglish
Pages (from-to)583-593
Number of pages11
JournalSensors and Actuators, B: Chemical
Volume123
Issue number1
DOIs
Publication statusPublished - Apr 10 2007

Fingerprint

Immunosensors
2,4-Dichlorophenoxyacetic Acid
Ovalbumin
Surface plasmon resonance
surface plasmon resonance
Monolayers
Atomic force microscopy
atomic force microscopy
acids
Acids
sensors
Sensors
chips
antibodies
Assays
Antibodies
enzymes
Enzymes
reactivity
buffers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Novel surface plasmon resonance (SPR) immunosensor based on monomolecular layer of physically-adsorbed ovalbumin conjugate for detection of 2,4-dichlorophenoxyacetic acid and atomic force microscopy study. / Gobi, K. Vengatajalabathy; Kim, Sook Jin; Tanaka, Hiroyuki; Shoyama, Yukihiro; Miura, Norio.

In: Sensors and Actuators, B: Chemical, Vol. 123, No. 1, 10.04.2007, p. 583-593.

Research output: Contribution to journalArticle

@article{5dd9f8c180084943942c50b02e38dbca,
title = "Novel surface plasmon resonance (SPR) immunosensor based on monomolecular layer of physically-adsorbed ovalbumin conjugate for detection of 2,4-dichlorophenoxyacetic acid and atomic force microscopy study",
abstract = "A rapid and simple optical immunosensor for detection of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has been developed based on SPR technology. Functional sensing surface of the immunosensor is created by immobilizing an ovalbumin conjugate of 2,4-D (2,4-D-OVA) by simple physical adsorption on an SPR thin-film gold chip. It has been established that the Au surface of the sensor chip was completely covered by 2,4-D-OVA up to a monomolecular layer and that the 2,4-D-OVA immobilized sensor chip was highly resistive to non-specific binding of proteins. Selective binding of a monoclonal antibody against 2,4-D (2,4-D-Ab) is followed by an increase in SPR angle. The antibody complexed on the sensor surface could be removed simply by the flow of an acidic buffer (glycine.HCl; 0.2 M, pH 2.0) for less than 1 min, facilitating repeated use of a same sensor chip. A competitive immunosensing method has been applied for the detection of 2,4-D, in which binding of the antibody onto the sensor surface in the presence and absence of 2,4-D is investigated. When 2,4-D is present in sample solution, a competition is set off between 2,4-D in solution and 2,4-D-OVA conjugate on sensor chip for binding to 2,4-D-Ab. A lowest detection limit of 0.1 ng/ml 2,4-D is established. Calibration curve of this analytical system covers a wide concentration range of 0.1-300 ng/ml 2,4-D. One assay could be completed in 18 min (binding, 15 min; acidic eluent and followed carrier buffer, 3 min). Enzyme-linked immunosorbent assay (ELISA) measurements for the detection of 2,4-D using analogous antigen-coat format showed a detection limit of 500 ng/ml. The high sensitivity of the configured SPR immunosensor system and the differences between the performances of SPR and ELISA are discussed. Cross-reactivity of the SPR sensor against a few compounds structurally and environmentally relevant to 2,4-D is examined. The fabricated SPR sensor is found to be highly resistant to interference with a maximum cross-reactivity of only 4{\%} for 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).",
author = "Gobi, {K. Vengatajalabathy} and Kim, {Sook Jin} and Hiroyuki Tanaka and Yukihiro Shoyama and Norio Miura",
year = "2007",
month = "4",
day = "10",
doi = "10.1016/j.snb.2006.09.056",
language = "English",
volume = "123",
pages = "583--593",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Novel surface plasmon resonance (SPR) immunosensor based on monomolecular layer of physically-adsorbed ovalbumin conjugate for detection of 2,4-dichlorophenoxyacetic acid and atomic force microscopy study

AU - Gobi, K. Vengatajalabathy

AU - Kim, Sook Jin

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

AU - Miura, Norio

PY - 2007/4/10

Y1 - 2007/4/10

N2 - A rapid and simple optical immunosensor for detection of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has been developed based on SPR technology. Functional sensing surface of the immunosensor is created by immobilizing an ovalbumin conjugate of 2,4-D (2,4-D-OVA) by simple physical adsorption on an SPR thin-film gold chip. It has been established that the Au surface of the sensor chip was completely covered by 2,4-D-OVA up to a monomolecular layer and that the 2,4-D-OVA immobilized sensor chip was highly resistive to non-specific binding of proteins. Selective binding of a monoclonal antibody against 2,4-D (2,4-D-Ab) is followed by an increase in SPR angle. The antibody complexed on the sensor surface could be removed simply by the flow of an acidic buffer (glycine.HCl; 0.2 M, pH 2.0) for less than 1 min, facilitating repeated use of a same sensor chip. A competitive immunosensing method has been applied for the detection of 2,4-D, in which binding of the antibody onto the sensor surface in the presence and absence of 2,4-D is investigated. When 2,4-D is present in sample solution, a competition is set off between 2,4-D in solution and 2,4-D-OVA conjugate on sensor chip for binding to 2,4-D-Ab. A lowest detection limit of 0.1 ng/ml 2,4-D is established. Calibration curve of this analytical system covers a wide concentration range of 0.1-300 ng/ml 2,4-D. One assay could be completed in 18 min (binding, 15 min; acidic eluent and followed carrier buffer, 3 min). Enzyme-linked immunosorbent assay (ELISA) measurements for the detection of 2,4-D using analogous antigen-coat format showed a detection limit of 500 ng/ml. The high sensitivity of the configured SPR immunosensor system and the differences between the performances of SPR and ELISA are discussed. Cross-reactivity of the SPR sensor against a few compounds structurally and environmentally relevant to 2,4-D is examined. The fabricated SPR sensor is found to be highly resistant to interference with a maximum cross-reactivity of only 4% for 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).

AB - A rapid and simple optical immunosensor for detection of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has been developed based on SPR technology. Functional sensing surface of the immunosensor is created by immobilizing an ovalbumin conjugate of 2,4-D (2,4-D-OVA) by simple physical adsorption on an SPR thin-film gold chip. It has been established that the Au surface of the sensor chip was completely covered by 2,4-D-OVA up to a monomolecular layer and that the 2,4-D-OVA immobilized sensor chip was highly resistive to non-specific binding of proteins. Selective binding of a monoclonal antibody against 2,4-D (2,4-D-Ab) is followed by an increase in SPR angle. The antibody complexed on the sensor surface could be removed simply by the flow of an acidic buffer (glycine.HCl; 0.2 M, pH 2.0) for less than 1 min, facilitating repeated use of a same sensor chip. A competitive immunosensing method has been applied for the detection of 2,4-D, in which binding of the antibody onto the sensor surface in the presence and absence of 2,4-D is investigated. When 2,4-D is present in sample solution, a competition is set off between 2,4-D in solution and 2,4-D-OVA conjugate on sensor chip for binding to 2,4-D-Ab. A lowest detection limit of 0.1 ng/ml 2,4-D is established. Calibration curve of this analytical system covers a wide concentration range of 0.1-300 ng/ml 2,4-D. One assay could be completed in 18 min (binding, 15 min; acidic eluent and followed carrier buffer, 3 min). Enzyme-linked immunosorbent assay (ELISA) measurements for the detection of 2,4-D using analogous antigen-coat format showed a detection limit of 500 ng/ml. The high sensitivity of the configured SPR immunosensor system and the differences between the performances of SPR and ELISA are discussed. Cross-reactivity of the SPR sensor against a few compounds structurally and environmentally relevant to 2,4-D is examined. The fabricated SPR sensor is found to be highly resistant to interference with a maximum cross-reactivity of only 4% for 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).

UR - http://www.scopus.com/inward/record.url?scp=33947688116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947688116&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2006.09.056

DO - 10.1016/j.snb.2006.09.056

M3 - Article

VL - 123

SP - 583

EP - 593

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 1

ER -