Novel tissue units of regional differentiation in the gut epithelium of Drosopbila, as revealed by P-element-mediated detection of enhancer

Ryutaro Murakami, Ayako Shigenaga, 1Akira Matsumoto, Ikuo Yamaoka, Teiichi Tanimura

Research output: Contribution to journalArticle

32 Citations (Scopus)


We analysed spatial patterns of expression of a lacZ reporter gene in the gut of Drosophila larvae that had been transformed with a P-element-lacZ vector to identify regional differences in gene expression. lacZ-positive epithelial cells formed distinct domains with discrete transverse and longitudinal boundaries along the gut tube. Boundaries were often found at sites at which morphological boundaries were not obvious. The gut epithelium was subdivided into 36 compartments by the boundaries. We refer to these novel compartments as "tissue compartments". The lacZ-positive domain of each strain appeared as a single tissue compartment or as a combination of several tissue compartments. The tissue compartment is considered to be a unit of regional differentiation. The spatial organization of the tissue compartments may represent the "floor plan", determined by genes that control the regional differentiation of this nonsegmental organ.

Original languageEnglish
Pages (from-to)243-249
Number of pages7
JournalRoux's Archives of Developmental Biology
Issue number5
Publication statusPublished - Mar 1 1994


All Science Journal Classification (ASJC) codes

  • Developmental Biology

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