NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network

Fumi Kano, Hisao Kondo, Akitsugu Yamamoto, Yayoi Kaneko, Keiji Uchiyama, Nobuko Hosokawa, Kazuhiro Nagata, Masayuki Murata

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The endoplasmic reticulum (ER) has a characteristic polygonal structure with hallmark three-way junctions. In a previous paper, we reconstituted the disruption of the pre-existing ER network using mitotic cytosol from HeLa cells in streptolysin O (SLO)-permeabilized CHO-HSP cells (stably expressing GFP-HSP47). In addition, we found that interphase cytosol induced reformation of the disrupted ER network into a continuous network structure. Here, we show that the reformation of the ER network is accomplished through two sequential fusion reactions. The first process is mediated by NSF/α and γ-SNAPs, and involves the generation of typical membranous intermediate structures that connect the disrupted ER tubules. A subsequent fusion is mediated by p97/p47/VCIP135, which has been shown to be required for homotypic fusion events in Golgi cisternae regrowth after mitosis. In addition, we also found that both fusion processes involve the t-SNARE, syntaxin 18.

Original languageEnglish
Pages (from-to)989-999
Number of pages11
JournalGenes to Cells
Volume10
Issue number10
DOIs
Publication statusPublished - Oct 1 2005

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Endoplasmic Reticulum
Cell Cycle
Cytosol
Qa-SNARE Proteins
SNARE Proteins
CHO Cells
Interphase
HeLa Cells
Mitosis

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network. / Kano, Fumi; Kondo, Hisao; Yamamoto, Akitsugu; Kaneko, Yayoi; Uchiyama, Keiji; Hosokawa, Nobuko; Nagata, Kazuhiro; Murata, Masayuki.

In: Genes to Cells, Vol. 10, No. 10, 01.10.2005, p. 989-999.

Research output: Contribution to journalArticle

Kano, Fumi ; Kondo, Hisao ; Yamamoto, Akitsugu ; Kaneko, Yayoi ; Uchiyama, Keiji ; Hosokawa, Nobuko ; Nagata, Kazuhiro ; Murata, Masayuki. / NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network. In: Genes to Cells. 2005 ; Vol. 10, No. 10. pp. 989-999.
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