Nuclear export signal in CDC25B

Sanae Uchida, Motoaki Ohtsubo, Mari Shimura, Masato Hirata, Hitoshi Nakagama, Tsukasa Matsunaga, Minoru Yoshida, Yukihito Ishizaka, Katsumi Yamashita

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13 Citations (Scopus)

Abstract

CDC25B is a dual-specificity phosphatase that activates CDK1/cyclin B. The nuclear exclusion of CDC25B is controlled by the binding of 14-3-3 to the nuclear export signal (NES) of CDC25B, which was reported to be amino acids H28 to L40 in the N-terminal region of CDC25B. In studying the subcellular localization of CDC25B, we found a functional NES at V52 to L65, the sequence of which is VTTLTQTMHDLAGL, where bold letters are leucine or hydrophobic amino acids frequently seen in an NES. The deletion of this NES sequence caused the mutant protein to locate exclusively in nuclei, while NES-fused GFP was detected in the cytoplasm. Moreover, the introduction of point mutations at some of the critical amino acids impaired cytoplasmic localization. Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. From these results, we concluded that the sequence we found is a bona fide NES of CDC25B.

Original languageEnglish
Pages (from-to)226-232
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number1
DOIs
Publication statusPublished - Mar 26 2004

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Uchida, S., Ohtsubo, M., Shimura, M., Hirata, M., Nakagama, H., Matsunaga, T., Yoshida, M., Ishizaka, Y., & Yamashita, K. (2004). Nuclear export signal in CDC25B. Biochemical and Biophysical Research Communications, 316(1), 226-232. https://doi.org/10.1016/j.bbrc.2004.02.039