Nuclear‐magnetic‐resonance studies of human epidermal growth factor

Robert M. COOKE, Michael J. TAPPIN, Iain D. CAMPBELL, Daisuke Kohda, Tetsuo MIYAKE, Toru FUWA, Tatsuo MIYAZAWA, Fuyuhiko INAGAKI

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The 1H‐NMR spectra of native human epidermal growth factor (EGF) and a derivative lacking the final five residues have been assigned by two‐dimensional methods, enabling their structures to be compared. The same structural features are observed for each protein, although the final five residues of native human EGF interact with residues earlier in the sequence. Comparison of the resonance shifts of human, rat and mouse EGF and human transforming growth factor α (TGFα) enables shifts characteristic of the EGF conformation to be identified, providing standards by which the structures of related proteins may be assessed.

Original languageEnglish
Pages (from-to)807-815
Number of pages9
JournalEuropean Journal of Biochemistry
Volume193
Issue number3
DOIs
Publication statusPublished - Jan 1 1990

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Epidermal Growth Factor
Population Groups
Transforming Growth Factors
Conformations
Rats
Proteins
Derivatives

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

COOKE, R. M., TAPPIN, M. J., CAMPBELL, I. D., Kohda, D., MIYAKE, T., FUWA, T., ... INAGAKI, F. (1990). Nuclear‐magnetic‐resonance studies of human epidermal growth factor. European Journal of Biochemistry, 193(3), 807-815. https://doi.org/10.1111/j.1432-1033.1990.tb19404.x

Nuclear‐magnetic‐resonance studies of human epidermal growth factor. / COOKE, Robert M.; TAPPIN, Michael J.; CAMPBELL, Iain D.; Kohda, Daisuke; MIYAKE, Tetsuo; FUWA, Toru; MIYAZAWA, Tatsuo; INAGAKI, Fuyuhiko.

In: European Journal of Biochemistry, Vol. 193, No. 3, 01.01.1990, p. 807-815.

Research output: Contribution to journalArticle

COOKE, RM, TAPPIN, MJ, CAMPBELL, ID, Kohda, D, MIYAKE, T, FUWA, T, MIYAZAWA, T & INAGAKI, F 1990, 'Nuclear‐magnetic‐resonance studies of human epidermal growth factor', European Journal of Biochemistry, vol. 193, no. 3, pp. 807-815. https://doi.org/10.1111/j.1432-1033.1990.tb19404.x
COOKE, Robert M. ; TAPPIN, Michael J. ; CAMPBELL, Iain D. ; Kohda, Daisuke ; MIYAKE, Tetsuo ; FUWA, Toru ; MIYAZAWA, Tatsuo ; INAGAKI, Fuyuhiko. / Nuclear‐magnetic‐resonance studies of human epidermal growth factor. In: European Journal of Biochemistry. 1990 ; Vol. 193, No. 3. pp. 807-815.
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