Nuclease tolerant FRET probe based on DNA-quantum dot conjugation

Daisuke Onoshima, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI). 2008

Original languageEnglish
Pages (from-to)181-183
Number of pages3
Journalanalytical sciences
Volume24
Issue number2
DOIs
Publication statusPublished - Jan 1 2008
Externally publishedYes

Fingerprint

Semiconductor quantum dots
DNA
Deoxyribonuclease EcoRI
DNA Restriction Enzymes
Electrostatics
Coloring Agents
Fluorescence Resonance Energy Transfer

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Nuclease tolerant FRET probe based on DNA-quantum dot conjugation. / Onoshima, Daisuke; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

In: analytical sciences, Vol. 24, No. 2, 01.01.2008, p. 181-183.

Research output: Contribution to journalArticle

Onoshima, Daisuke ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Nuclease tolerant FRET probe based on DNA-quantum dot conjugation. In: analytical sciences. 2008 ; Vol. 24, No. 2. pp. 181-183.
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