Nucleation-synchronized strand displacement for highly sensitive DNA analysis.

Kazuya Hirata, Yuichi Sato, Arihiro Kano, Toshihiro Akaike, Atsushi Maruyama

Research output: Contribution to journalArticle

Abstract

Nucleation process is a rate-limiting step of DNA strand exchange reactions between double helical DNA and its complementary DNA. Here we designed the double stranded probes that have single stranded portion. The single stranded portion is responsible for the nucleation process and mismatch recognition. A single base mismatch in 25mer DNAs could be detected in a few minutes at ambient temperatures. Using this method, various types of SNPs are rapidly and reliably detected without any aids of enzymes or a special instrument.

Original languageEnglish
Pages (from-to)267-268
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number48
Publication statusPublished - Jan 1 2004
Externally publishedYes

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DNA
Single Nucleotide Polymorphism
Complementary DNA
Temperature
Enzymes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Nucleation-synchronized strand displacement for highly sensitive DNA analysis. / Hirata, Kazuya; Sato, Yuichi; Kano, Arihiro; Akaike, Toshihiro; Maruyama, Atsushi.

In: Nucleic acids symposium series (2004), No. 48, 01.01.2004, p. 267-268.

Research output: Contribution to journalArticle

Hirata, Kazuya ; Sato, Yuichi ; Kano, Arihiro ; Akaike, Toshihiro ; Maruyama, Atsushi. / Nucleation-synchronized strand displacement for highly sensitive DNA analysis. In: Nucleic acids symposium series (2004). 2004 ; No. 48. pp. 267-268.
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