Nucleation-synchronized strand displacement for highly sensitive DNA analysis.

Kazuya Hirata; Yuichi Sato; Arihiro Kano; Toshihiro Akaike; Atsushi Maruyama

Nucleation-synchronized strand displacement for highly sensitive DNA analysis.

Kazuya Hirata, Yuichi Sato, Arihiro Kano, Toshihiro Akaike, Atsushi Maruyama

Research output: Contribution to journal › Article › peer-review

Abstract

Nucleation process is a rate-limiting step of DNA strand exchange reactions between double helical DNA and its complementary DNA. Here we designed the double stranded probes that have single stranded portion. The single stranded portion is responsible for the nucleation process and mismatch recognition. A single base mismatch in 25mer DNAs could be detected in a few minutes at ambient temperatures. Using this method, various types of SNPs are rapidly and reliably detected without any aids of enzymes or a special instrument.

Original language	English
Pages (from-to)	267-268
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	48
Publication status	Published - Jan 1 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Medicine(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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abstract = "Nucleation process is a rate-limiting step of DNA strand exchange reactions between double helical DNA and its complementary DNA. Here we designed the double stranded probes that have single stranded portion. The single stranded portion is responsible for the nucleation process and mismatch recognition. A single base mismatch in 25mer DNAs could be detected in a few minutes at ambient temperatures. Using this method, various types of SNPs are rapidly and reliably detected without any aids of enzymes or a special instrument.",

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AU - Sato, Yuichi

AU - Kano, Arihiro

AU - Akaike, Toshihiro

AU - Maruyama, Atsushi

PY - 2004/1/1

Y1 - 2004/1/1

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AB - Nucleation process is a rate-limiting step of DNA strand exchange reactions between double helical DNA and its complementary DNA. Here we designed the double stranded probes that have single stranded portion. The single stranded portion is responsible for the nucleation process and mismatch recognition. A single base mismatch in 25mer DNAs could be detected in a few minutes at ambient temperatures. Using this method, various types of SNPs are rapidly and reliably detected without any aids of enzymes or a special instrument.

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ER -

Nucleation-synchronized strand displacement for highly sensitive DNA analysis.

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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