Streptomyces nucleotide 3′-pyrophosphokinase, formerly known to synthesize a variety of 3′-pyrophospho nucleotides by 5′-β,γ-pyrophosphoryl transfer from ATP, ATP 3′-pyrophosphate, and dATP, can also synthesize 2′,3′-cyclic monophospho nucleotides using diadenosine 5′,5′-poly (tri, tetra, and penta) phosphates as the phosphate donor. The examples are pA > p, ppA > p, pG > p, and CpG > p. Transient formation of an -N(3′)pp(5′)A structure via ADP transfer from the donor followed by AMP elimination is proposed as their synthetic mechanism.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)