Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics

Ottilie Von Loeffelholz, Neil A. Venables, Douglas Robert Drummond, Miho Katsuki, Robert Cross, Carolyn A. Moores

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Using cryo-electron microscopy, we characterize the architecture of microtubules assembled from Schizosaccharomyces pombe tubulin, in the presence and absence of their regulatory partner Mal3. Cryo-electron tomography reveals that microtubules assembled from S. pombe tubulin have predominantly B-lattice interprotofilament contacts, with protofilaments skewed around the microtubule axis. Copolymerization with Mal3 favors 13 protofilament microtubules with reduced protofilament skew, indicating that Mal3 adjusts interprotofilament interfaces. A 4.6-Å resolution structure of microtubule-bound Mal3 shows that Mal3 makes a distinctive footprint on the S. pombe microtubule lattice and that unlike mammalian microtubules, S. pombe microtubules do not show the longitudinal lattice compaction associated with EB protein binding and GTP hydrolysis. Our results firmly support a structural plasticity view of microtubule dynamics in which microtubule lattice conformation is sensitive to a variety of effectors and differently so for different tubulins.

Original languageEnglish
Article number2241
JournalNature communications
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Schizosaccharomyces
yeast
nucleotides
Tubulin
plastic properties
Microtubules
Yeast
Plasticity
fission
Nucleotides
effectors
GTP-Binding Proteins
Copolymerization
Electron microscopy
Tomography
Conformations
Hydrolysis
Compaction
copolymerization
footprints

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics. / Von Loeffelholz, Ottilie; Venables, Neil A.; Drummond, Douglas Robert; Katsuki, Miho; Cross, Robert; Moores, Carolyn A.

In: Nature communications, Vol. 8, No. 1, 2241, 01.12.2017.

Research output: Contribution to journalArticle

Von Loeffelholz, Ottilie ; Venables, Neil A. ; Drummond, Douglas Robert ; Katsuki, Miho ; Cross, Robert ; Moores, Carolyn A. / Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics. In: Nature communications. 2017 ; Vol. 8, No. 1.
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