Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

Yoshizumi Ishino; Hideo Shinagawa; Kozo Makino; Susumu Tsunasawa; Fumio Sakiyama; Atsuo Nakata

doi:10.1007/BF00330179

Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

Yoshizumi Ishino, Hideo Shinagawa, Kozo Makino, Susumu Tsunasawa, Fumio Sakiyama, Atsuo Nakata

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	MGG Molecular & General Genetics
Volume	204
Issue number	1
DOIs	https://doi.org/10.1007/BF00330179
Publication status	Published - Jul 1986
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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10.1007/BF00330179

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abstract = "The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.",

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AU - Ishino, Yoshizumi

AU - Shinagawa, Hideo

AU - Makino, Kozo

AU - Tsunasawa, Susumu

AU - Sakiyama, Fumio

AU - Nakata, Atsuo

PY - 1986/7

Y1 - 1986/7

N2 - The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.

AB - The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.

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Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

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