Nucleotide sequence of the nbosomal RNA gene of physarum polycephalum: Intron 2 and its flanking regions of the 26S rRNA gene

Hisayuki Nomiyama, Satoru Kuhara, Toshio Kukita, Takeshi Otsuka, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The 26S ribosomal RNA gene of Physarum polycephalum is interrupted by two introns, and we have previously determined the sequence of one of them (intron 1) (Nomiyama et al. Proc.Natl.Acad.Sci.USA 78, 1376-1380, 1981). In this study we sequenced the second intron (intron 2) of about 0.5 kb length and its flanking regions, and found that one nucleotide at each junction is identical in intron 1 and intron 2, though the junction regions share no other sequence homology. Comparison of the flanking exon sequences to E. coli 23S rRNA sequences shows that conserved sequences are interspersed with tracts having little homology. In particular, the region encompassing the intron 2 interruption site is highly conserved. The E. coli ribosomal protein Ll binding region is also conserved.

Original languageEnglish
Pages (from-to)5507-5520
Number of pages14
JournalNucleic acids research
Volume9
Issue number21
DOIs
Publication statusPublished - Nov 11 1981

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Physarum polycephalum
rRNA Genes
Introns
RNA
Genes
Conserved Sequence
Ribosomal Proteins
Escherichia coli Proteins
Sequence Homology
ribosomal RNA 26S
Protein Binding
Exons
Nucleotides
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Nucleotide sequence of the nbosomal RNA gene of physarum polycephalum : Intron 2 and its flanking regions of the 26S rRNA gene. / Nomiyama, Hisayuki; Kuhara, Satoru; Kukita, Toshio; Otsuka, Takeshi; Sakaki, Yoshiyuki.

In: Nucleic acids research, Vol. 9, No. 21, 11.11.1981, p. 5507-5520.

Research output: Contribution to journalArticle

Nomiyama, Hisayuki ; Kuhara, Satoru ; Kukita, Toshio ; Otsuka, Takeshi ; Sakaki, Yoshiyuki. / Nucleotide sequence of the nbosomal RNA gene of physarum polycephalum : Intron 2 and its flanking regions of the 26S rRNA gene. In: Nucleic acids research. 1981 ; Vol. 9, No. 21. pp. 5507-5520.
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