Nucleotide variation and conservation at the dpp locus, a gene controlling early development in Drosophila

Brent Richter, Manyuan Long, R. C. Lewontin, Eiji Nitasaka

Research output: Contribution to journalReview article

32 Citations (Scopus)

Abstract

A study of polymorphism and species divergence of the dpp gene of Drosophila has been made. Eighteen lines from a population of D. melanogaster were sequenced for 5200 bp of the Hin region of the gene, coding for the dpp polypeptide. A comparison was made with sequence from D. simulans. Ninety- six silent polymorphisms and three amino acid replacement polymorphisms were found. The overall silent polymorphism (0.0247) is low, but haplotype diversity (0.0066 for effectively silent sites and 0.0054 for all sites) is in the range found for enzyme loci. Amino acid variation is absent in the N- terminal signal peptide, the C-terminal TGF-β peptide and in the N-terminal half of the pro-protein region. At the nucleotide level there is strong conservation in the middle half of the large intron and in the 3' untranslated sequence of the last exon. The 3' untranslated conservation, which is perfect for 110 bp among all the divergent species, is unexplained. There is strong positive linkage disequilibrium among polymorphic sites, with stretches of apparent gene conversion among originally divergent sequences. The population apparently is a migration of divergent clades.

Original languageEnglish
Pages (from-to)311-323
Number of pages13
JournalGenetics
Volume145
Issue number2
Publication statusPublished - Feb 1 1997

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Drosophila
Nucleotides
Amino Acids
Gene Conversion
Peptides
Linkage Disequilibrium
Protein Sorting Signals
Introns
Haplotypes
Population
Genes
Exons
Enzymes
Proteins
polypeptide C

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Nucleotide variation and conservation at the dpp locus, a gene controlling early development in Drosophila. / Richter, Brent; Long, Manyuan; Lewontin, R. C.; Nitasaka, Eiji.

In: Genetics, Vol. 145, No. 2, 01.02.1997, p. 311-323.

Research output: Contribution to journalReview article

Richter, Brent ; Long, Manyuan ; Lewontin, R. C. ; Nitasaka, Eiji. / Nucleotide variation and conservation at the dpp locus, a gene controlling early development in Drosophila. In: Genetics. 1997 ; Vol. 145, No. 2. pp. 311-323.
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