Nutrient starvation differentially regulates the autophagy-related gene GmATG8i in soybean seedlings

Nang Myint Phyu Sin Htwe, Hiroyuki Tanigawa, Yushi Ishibashi, Shao Hui Zheng, Takashi Yuasa, Mari Iwaya-Inoue

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Autophagy functions in bulk degradation of proteins and organelles for nutrient recycling. Recent evidence suggests that a set of autophagy-related (ATG) genes are induced under starvation, senescence and various abiotic stresses. To understand how these genes function in plants, expression profiles of soybean ATG homologs, GmATG8s, GmATG4, GmATG9, GmATG12 and GmATG18a, were examined using seedlings of Glycine max (L.) Merr. cv. Fukuyutaka) subjected to various nutrient conditions. Among them, GmATG8i mRNA was induced at higher level than any other ATGs under starvation. Immuoblot with a specific antibody raised against GmAtg8i indicated that endogenous GmAtg8i associates to microsomes of soybean seedling extracts. In the presence of vacuolar protease inhibitors, significant accumulation of anti-GmAtg8i was observed and mRNA level of GmATG8i increased in higher level than in the absence of the inhibitors. These results suggest that in soybean gene expression of ATG8i is regulated by both environmental nutrient conditions and intracellular nutrient recycling via proteolysis in vacuole.

Original languageEnglish
Pages (from-to)317-326
Number of pages10
JournalPlant Biotechnology
Volume26
Issue number3
DOIs
Publication statusPublished - Jan 1 2009

Fingerprint

autophagy
Autophagy
Starvation
Seedlings
Soybeans
starvation
soybeans
Food
seedlings
nutrients
Proteolysis
Genes
recycling
genes
Messenger RNA
microsomes
Microsomes
protein degradation
Vacuoles
proteinase inhibitors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Nutrient starvation differentially regulates the autophagy-related gene GmATG8i in soybean seedlings. / Htwe, Nang Myint Phyu Sin; Tanigawa, Hiroyuki; Ishibashi, Yushi; Zheng, Shao Hui; Yuasa, Takashi; Iwaya-Inoue, Mari.

In: Plant Biotechnology, Vol. 26, No. 3, 01.01.2009, p. 317-326.

Research output: Contribution to journalArticle

Htwe, Nang Myint Phyu Sin ; Tanigawa, Hiroyuki ; Ishibashi, Yushi ; Zheng, Shao Hui ; Yuasa, Takashi ; Iwaya-Inoue, Mari. / Nutrient starvation differentially regulates the autophagy-related gene GmATG8i in soybean seedlings. In: Plant Biotechnology. 2009 ; Vol. 26, No. 3. pp. 317-326.
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