Nutrition-adaptive control of multiple-bacteriocin production by Weissella hellenica QU 13

Y. Masuda, R. H. Perez, T. Zendo, K. Sonomoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aim: To analyse nutrition-adaptive multiple-bacteriocin production by Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13 produces two leaderless bacteriocins, weissellicins Y and M. Their production was studied in MRS and APT media by quantification analyses with liquid chromatography mass spectrometry (LC/MS), while transcriptional analysis of biosynthetic genes was performed by real-time reverse transcription (RT)-PCR. Weissellicin Y production was higher in MRS culture than in APT culture, while weissellicin M production was higher in APT culture than in MRS culture. APT medium contains a higher amount of thiamine than MRS medium, to enhance the growth of heterofermentative lactic acid bacteria. Therefore, thiamine addition to MRS culture enhanced the growth of W. hellenica QU 13; consequently, weissellicin Y production was decreased, while weissellicin M production was not affected. Furthermore, real-time RT-PCR analyses indicated that the transcriptional trends of their respective structural genes, welY and welM, were different from each other, and that these two genes' transcriptions responded to nutrition conditions. Conclusion: Weissella hellenica QU 13 was demonstrated to control weissellicins Y and M production based on nutrition conditions. In addition, differential expression behaviour of weissellicins Y and M indicates that each of them would have separate roles to adapt to different environmental situations. Significance and Impact of the Study: This is the first report that describes nutrition-adaptive multiple-bacteriocin production, in which thiamine inhibits bacteriocin production while it enhances the growth of the producer strain.

Original languageEnglish
Pages (from-to)70-79
Number of pages10
JournalJournal of Applied Microbiology
Volume120
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

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Weissella
Bacteriocins
Thiamine
Reverse Transcription
Growth
Genes
Polymerase Chain Reaction
Liquid Chromatography
Lactic Acid
Mass Spectrometry
Bacteria
APT

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Nutrition-adaptive control of multiple-bacteriocin production by Weissella hellenica QU 13. / Masuda, Y.; Perez, R. H.; Zendo, T.; Sonomoto, K.

In: Journal of Applied Microbiology, Vol. 120, No. 1, 01.01.2016, p. 70-79.

Research output: Contribution to journalArticle

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abstract = "Aim: To analyse nutrition-adaptive multiple-bacteriocin production by Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13 produces two leaderless bacteriocins, weissellicins Y and M. Their production was studied in MRS and APT media by quantification analyses with liquid chromatography mass spectrometry (LC/MS), while transcriptional analysis of biosynthetic genes was performed by real-time reverse transcription (RT)-PCR. Weissellicin Y production was higher in MRS culture than in APT culture, while weissellicin M production was higher in APT culture than in MRS culture. APT medium contains a higher amount of thiamine than MRS medium, to enhance the growth of heterofermentative lactic acid bacteria. Therefore, thiamine addition to MRS culture enhanced the growth of W. hellenica QU 13; consequently, weissellicin Y production was decreased, while weissellicin M production was not affected. Furthermore, real-time RT-PCR analyses indicated that the transcriptional trends of their respective structural genes, welY and welM, were different from each other, and that these two genes' transcriptions responded to nutrition conditions. Conclusion: Weissella hellenica QU 13 was demonstrated to control weissellicins Y and M production based on nutrition conditions. In addition, differential expression behaviour of weissellicins Y and M indicates that each of them would have separate roles to adapt to different environmental situations. Significance and Impact of the Study: This is the first report that describes nutrition-adaptive multiple-bacteriocin production, in which thiamine inhibits bacteriocin production while it enhances the growth of the producer strain.",
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