Abstract
The effects of nystatin, a polyene antibiotic, was studied in Saccharomyces cerevisiae by isolating and characterizing nystatin-sensitive mutants. We isolated a number of nystatin-sensitive mutants by ethylmethane sulfonate mutagenesis. One of these mutants, the nss1 mutant, was characterized in detail. The mutant was sensitive to stresses such as high temperature or high concentrations of monovalent and divalent cations. The nss1 mutants showed severe vacuolar protein sorting and vacuolar morphology defects. The nss1 mutant was demonstrated to have a mutational lesion in the known VPS16 gene, which is essential for vacuolar protein sorting in S. cerevisiae. All of the vacuolar deficient mutants (vps11, vps16, vps18, and vps33) were sensitive to nystatin. Nystatin was found to cause extensive enlargement of the vacuole in wild-type S. cerevisiae cells. These results are discussed with special reference to the vacuolar function of S. cerevisiae.
| Original language | English |
|---|---|
| Pages (from-to) | 1075-1082 |
| Number of pages | 8 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 63 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jan 1 1999 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Nystatin effects on vacuolar function in saccharomyce. / Bhuiyan, M. Shah Alam; Ito, Yuji; Nakamura, Atsuko; Tanaka, Naotaka; Fujita, Kiyotaka; Fukui, Hiroshi; Takegawa, Kaoru.
In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 6, 01.01.1999, p. 1075-1082.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nystatin effects on vacuolar function in saccharomyce
AU - Bhuiyan, M. Shah Alam
AU - Ito, Yuji
AU - Nakamura, Atsuko
AU - Tanaka, Naotaka
AU - Fujita, Kiyotaka
AU - Fukui, Hiroshi
AU - Takegawa, Kaoru
PY - 1999/1/1
Y1 - 1999/1/1
N2 - The effects of nystatin, a polyene antibiotic, was studied in Saccharomyces cerevisiae by isolating and characterizing nystatin-sensitive mutants. We isolated a number of nystatin-sensitive mutants by ethylmethane sulfonate mutagenesis. One of these mutants, the nss1 mutant, was characterized in detail. The mutant was sensitive to stresses such as high temperature or high concentrations of monovalent and divalent cations. The nss1 mutants showed severe vacuolar protein sorting and vacuolar morphology defects. The nss1 mutant was demonstrated to have a mutational lesion in the known VPS16 gene, which is essential for vacuolar protein sorting in S. cerevisiae. All of the vacuolar deficient mutants (vps11, vps16, vps18, and vps33) were sensitive to nystatin. Nystatin was found to cause extensive enlargement of the vacuole in wild-type S. cerevisiae cells. These results are discussed with special reference to the vacuolar function of S. cerevisiae.
AB - The effects of nystatin, a polyene antibiotic, was studied in Saccharomyces cerevisiae by isolating and characterizing nystatin-sensitive mutants. We isolated a number of nystatin-sensitive mutants by ethylmethane sulfonate mutagenesis. One of these mutants, the nss1 mutant, was characterized in detail. The mutant was sensitive to stresses such as high temperature or high concentrations of monovalent and divalent cations. The nss1 mutants showed severe vacuolar protein sorting and vacuolar morphology defects. The nss1 mutant was demonstrated to have a mutational lesion in the known VPS16 gene, which is essential for vacuolar protein sorting in S. cerevisiae. All of the vacuolar deficient mutants (vps11, vps16, vps18, and vps33) were sensitive to nystatin. Nystatin was found to cause extensive enlargement of the vacuole in wild-type S. cerevisiae cells. These results are discussed with special reference to the vacuolar function of S. cerevisiae.
UR - http://www.scopus.com/inward/record.url?scp=0033146105&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033146105&partnerID=8YFLogxK
U2 - 10.1271/bbb.63.1075
DO - 10.1271/bbb.63.1075
M3 - Article
C2 - 10427694
AN - SCOPUS:0033146105
VL - 63
SP - 1075
EP - 1082
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 6
ER -