TY - JOUR
T1 - Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose
AU - Kumagai, Keiko
AU - Akakabe, Mai
AU - Tsuda, Masayuki
AU - Tsuda, Masashi
AU - Fukushi, Eri
AU - Kawabata, Jun
AU - Abe, Takamasa
AU - Ichikawa, Kazuhiro
N1 - Funding Information:
We thank Prof. S. Sando and Dr. H. Nonaka of Kyushu University for methodological advise of cell lysate experiments, Prof. A Tominaga and Dr. Y. Konishi of Kochi University for kindly gift of THP cells and technical support of cell cultivation, Dr. J. Kurita and T. Hino of Agilent Technologies Japan Ltd. for assistance of array NMR measurements, and S. Goto of Oxford Instruments KK for assistance for DNP operation. This study was partially supported by a Grant-in-Aid for Young Scientists (B) (Grant No. 25860079) from Japan Society for the Promotion of Science. K.I. was in part supported by the funding program 'Creation of Innovation Centers for Advanced Interdisciplinary Research Areas' from JST, commissioned by MEXT.
Funding Information:
Acknowledgments We thank Prof. S. Sando and Dr. H. Nonaka of Kyushu University for methodological advise of cell lysate experiments, Prof. A Tominaga and Dr. Y. Konishi of Kochi University for kindly gift of THP cells and technical support of cell cultivation, Dr. J. Kurita and T. Hino of Agilent Technologies Japan Ltd. for assistance of array NMR measurements, and S. Goto of Oxford Instruments KK for assistance for DNP operation. This study was partially supported by a Grant-in-Aid for Young Scientists (B) (Grant No. 25860079) from Japan Society for the Promotion of Science. K.I. was in part supported by the funding program ‘Creation of Innovation Centers for Advanced Interdisciplinary Research Areas’ from JST, commissioned by MEXT.
Publisher Copyright:
© 2014 The Pharmaceutical Society of Japan.
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.
AB - Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.
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U2 - 10.1248/bpb.b14-00156
DO - 10.1248/bpb.b14-00156
M3 - Article
C2 - 25087964
AN - SCOPUS:84906775858
SN - 0918-6158
VL - 37
SP - 1416
EP - 1421
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 8
ER -