Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose

Keiko Kumagai, Mai Akakabe, Masayuki Tsuda, Masashi Tsuda, Eri Fukushi, Jun Kawabata, Takamasa Abe, Kazuhiro Ichikawa

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.

    Original languageEnglish
    Pages (from-to)1416-1421
    Number of pages6
    JournalBiological and Pharmaceutical Bulletin
    Volume37
    Issue number8
    DOIs
    Publication statusPublished - Aug 1 2014

    All Science Journal Classification (ASJC) codes

    • Pharmacology
    • Pharmaceutical Science

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