Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose

Keiko Kumagai, Mai Akakabe, Masayuki Tsuda, Masashi Tsuda, Eri Fukushi, Jun Kawabata, Takamasa Abe, Kazuhiro Ichikawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.

Original languageEnglish
Pages (from-to)1416-1421
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume37
Issue number8
DOIs
Publication statusPublished - Aug 1 2014

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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    Kumagai, K., Akakabe, M., Tsuda, M., Tsuda, M., Fukushi, E., Kawabata, J., Abe, T., & Ichikawa, K. (2014). Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose. Biological and Pharmaceutical Bulletin, 37(8), 1416-1421. https://doi.org/10.1248/bpb.b14-00156