Observation of hybridization on a DNA array by surface plasmon resonace imaging using Au nanoparticles

Fumio Nakamura; Masateru Ito; Ablihito Manna; Kaoru Tamada; Masahiko Hara; Wolfgang Knoll

doi:10.1143/JJAP.45.1026

Observation of hybridization on a DNA array by surface plasmon resonace imaging using Au nanoparticles

Fumio Nakamura, Masateru Ito, Ablihito Manna, Kaoru Tamada, Masahiko Hara, Wolfgang Knoll

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

To realize the sensitive detection of DNA hybridization, DNA-modified Au nanoparticles (DNA-AuNPs) containing single-and double-stranded portions were prepared. The hybridization of a target DNA using the DNA-AuNPs on a DNA self-assembled monolayer (DNA-SAM) was monitored in situ by surface plasmon resonance (SPR) imaging. A sandwich type hybridization (DNA-SAM/target DNA/DNA-AuNP) experiment was carried out in order to enhance the hybridization signal. SPR imaging results strongly indicate that the hybridization signal is enhanced several times compared to the case of target DNA hybridization.

Original language	English
Pages (from-to)	1026-1029
Number of pages	4
Journal	Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume	45
Issue number	2 A
DOIs	https://doi.org/10.1143/JJAP.45.1026
Publication status	Published - Feb 8 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Engineering(all)
Physics and Astronomy(all)

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10.1143/JJAP.45.1026

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Observation of hybridization on a DNA array by surface plasmon resonace imaging using Au nanoparticles. / Nakamura, Fumio; Ito, Masateru; Manna, Ablihito et al.
In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 45, No. 2 A, 08.02.2006, p. 1026-1029.

Research output: Contribution to journal › Article › peer-review

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AU - Knoll, Wolfgang

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AB - To realize the sensitive detection of DNA hybridization, DNA-modified Au nanoparticles (DNA-AuNPs) containing single-and double-stranded portions were prepared. The hybridization of a target DNA using the DNA-AuNPs on a DNA self-assembled monolayer (DNA-SAM) was monitored in situ by surface plasmon resonance (SPR) imaging. A sandwich type hybridization (DNA-SAM/target DNA/DNA-AuNP) experiment was carried out in order to enhance the hybridization signal. SPR imaging results strongly indicate that the hybridization signal is enhanced several times compared to the case of target DNA hybridization.

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Observation of hybridization on a DNA array by surface plasmon resonace imaging using Au nanoparticles

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