Observation of hybridization on a DNA array by surface plasmon resonace imaging using Au nanoparticles

Fumio Nakamura, Masateru Ito, Ablihito Manna, Kaoru Tamada, Masahiko Hara, Wolfgang Knoll

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To realize the sensitive detection of DNA hybridization, DNA-modified Au nanoparticles (DNA-AuNPs) containing single-and double-stranded portions were prepared. The hybridization of a target DNA using the DNA-AuNPs on a DNA self-assembled monolayer (DNA-SAM) was monitored in situ by surface plasmon resonance (SPR) imaging. A sandwich type hybridization (DNA-SAM/target DNA/DNA-AuNP) experiment was carried out in order to enhance the hybridization signal. SPR imaging results strongly indicate that the hybridization signal is enhanced several times compared to the case of target DNA hybridization.

Original languageEnglish
Pages (from-to)1026-1029
Number of pages4
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume45
Issue number2 A
DOIs
Publication statusPublished - Feb 8 2006
Externally publishedYes

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DNA
deoxyribonucleic acid
Nanoparticles
Imaging techniques
nanoparticles
Surface plasmon resonance
Self assembled monolayers
surface plasmon resonance
Experiments

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Observation of hybridization on a DNA array by surface plasmon resonace imaging using Au nanoparticles. / Nakamura, Fumio; Ito, Masateru; Manna, Ablihito; Tamada, Kaoru; Hara, Masahiko; Knoll, Wolfgang.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 45, No. 2 A, 08.02.2006, p. 1026-1029.

Research output: Contribution to journalArticle

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