Observation of on-chip electrophoresis microcapillary using confocal laser scanning microscopy

S. Etoh, T. Fujimura, R. Hattori, Y. Kuroki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In a capillary electrophoresis separation performance is affected by various factors, for example, an electroosmotic flow, dispersion of sample plug and sample adsorption to capillary wall. In order to miniaturize the microcapillary onto a chip we have to understand them in detail and investigate their influence. Confocal Laser Scanning Microscopy (CLSM) in fluorescence mode (FV300 Fluoview OLYMPUS JAPAN CO., LTD, Japan) was used to observe the motion of the samples in microcapillary fabricated on a chip. The CLSM is a powerful tool to understand their influence since it can capture the fluorescence images sliced in the plane perpendicular to the optical axis and can scan the plane position along the optical axis. Therefore it makes possible to observe the sample behavior inside the channel. This study also contributes the investigation of the microfluidic manipulation which includes a micropump, a microvalve and other microfluidic actuator devices under electrokinetic driven conditions.

Original languageEnglish
Title of host publication2002 International Microprocesses and Nanotechnology Conference, MNC 2002
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages172-173
Number of pages2
ISBN (Electronic)4891140313, 9784891140311
DOIs
Publication statusPublished - Jan 1 2002
EventInternational Microprocesses and Nanotechnology Conference, MNC 2002 - Tokyo, Japan
Duration: Nov 6 2002Nov 8 2002

Publication series

Name2002 International Microprocesses and Nanotechnology Conference, MNC 2002

Other

OtherInternational Microprocesses and Nanotechnology Conference, MNC 2002
CountryJapan
CityTokyo
Period11/6/0211/8/02

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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