Observation of Transmitted Light Spectra during Gelation Process of Actomyosin

Haruichi Kanaya, Kazuhiro Hara, Hirotaka Okabe, Kazumi Matsushige, So Nishimuta, Michio Muguruma, Toshiyuki Fukazawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The heat-induced gelation process of muscle protein, actomyosin, was investigated by observing the transmitted light spectra. First, the temperature characteristic of the gelation (T'g) was determined from the spectral changes, showing remarkable dependence on pH and [NaCl]. Moreover, the spectra varied drastically with time after the temperature-jump procedure. From the time dependence of the observed spectra, the turbidity was calculated to characterize the light-scattering properties. The logarithm of the turbidity showed approximately linear dependence on the logarithm of the wavelength, and the slope increased with time, suggesting the growth of the actomyosin assembly during the gelation process.

Original languageEnglish
Pages (from-to)1113-1118
Number of pages6
JournalJournal of the Physical Society of Japan
Volume61
Issue number3
DOIs
Publication statusPublished - 1992

Fingerprint

gelation
turbidity
logarithms
muscles
time dependence
light scattering
assembly
slopes
proteins
heat
temperature
wavelengths

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

Cite this

Observation of Transmitted Light Spectra during Gelation Process of Actomyosin. / Kanaya, Haruichi; Hara, Kazuhiro; Okabe, Hirotaka; Matsushige, Kazumi; Nishimuta, So; Muguruma, Michio; Fukazawa, Toshiyuki.

In: Journal of the Physical Society of Japan, Vol. 61, No. 3, 1992, p. 1113-1118.

Research output: Contribution to journalArticle

Kanaya, Haruichi ; Hara, Kazuhiro ; Okabe, Hirotaka ; Matsushige, Kazumi ; Nishimuta, So ; Muguruma, Michio ; Fukazawa, Toshiyuki. / Observation of Transmitted Light Spectra during Gelation Process of Actomyosin. In: Journal of the Physical Society of Japan. 1992 ; Vol. 61, No. 3. pp. 1113-1118.
@article{889efeed0a4342328b2f545f6af57d6d,
title = "Observation of Transmitted Light Spectra during Gelation Process of Actomyosin",
abstract = "The heat-induced gelation process of muscle protein, actomyosin, was investigated by observing the transmitted light spectra. First, the temperature characteristic of the gelation (T'g) was determined from the spectral changes, showing remarkable dependence on pH and [NaCl]. Moreover, the spectra varied drastically with time after the temperature-jump procedure. From the time dependence of the observed spectra, the turbidity was calculated to characterize the light-scattering properties. The logarithm of the turbidity showed approximately linear dependence on the logarithm of the wavelength, and the slope increased with time, suggesting the growth of the actomyosin assembly during the gelation process.",
author = "Haruichi Kanaya and Kazuhiro Hara and Hirotaka Okabe and Kazumi Matsushige and So Nishimuta and Michio Muguruma and Toshiyuki Fukazawa",
year = "1992",
doi = "10.1143/JPSJ.61.1113",
language = "English",
volume = "61",
pages = "1113--1118",
journal = "Journal of the Physical Society of Japan",
issn = "0031-9015",
publisher = "Physical Society of Japan",
number = "3",

}

TY - JOUR

T1 - Observation of Transmitted Light Spectra during Gelation Process of Actomyosin

AU - Kanaya, Haruichi

AU - Hara, Kazuhiro

AU - Okabe, Hirotaka

AU - Matsushige, Kazumi

AU - Nishimuta, So

AU - Muguruma, Michio

AU - Fukazawa, Toshiyuki

PY - 1992

Y1 - 1992

N2 - The heat-induced gelation process of muscle protein, actomyosin, was investigated by observing the transmitted light spectra. First, the temperature characteristic of the gelation (T'g) was determined from the spectral changes, showing remarkable dependence on pH and [NaCl]. Moreover, the spectra varied drastically with time after the temperature-jump procedure. From the time dependence of the observed spectra, the turbidity was calculated to characterize the light-scattering properties. The logarithm of the turbidity showed approximately linear dependence on the logarithm of the wavelength, and the slope increased with time, suggesting the growth of the actomyosin assembly during the gelation process.

AB - The heat-induced gelation process of muscle protein, actomyosin, was investigated by observing the transmitted light spectra. First, the temperature characteristic of the gelation (T'g) was determined from the spectral changes, showing remarkable dependence on pH and [NaCl]. Moreover, the spectra varied drastically with time after the temperature-jump procedure. From the time dependence of the observed spectra, the turbidity was calculated to characterize the light-scattering properties. The logarithm of the turbidity showed approximately linear dependence on the logarithm of the wavelength, and the slope increased with time, suggesting the growth of the actomyosin assembly during the gelation process.

UR - http://www.scopus.com/inward/record.url?scp=77953076549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953076549&partnerID=8YFLogxK

U2 - 10.1143/JPSJ.61.1113

DO - 10.1143/JPSJ.61.1113

M3 - Article

VL - 61

SP - 1113

EP - 1118

JO - Journal of the Physical Society of Japan

JF - Journal of the Physical Society of Japan

SN - 0031-9015

IS - 3

ER -