Occurrence of a novel fucose-containing pentaglycosylceramide with blood-group-B active determinant in Xenopus blastula cells

Its possible involvement in cell-cell adhesion

Kazuya Nomura, Nobushige Nakajo, K. L.P.J. Hidari, H. Nomura, M. Murata, M. Suzuki, K. Yamana, Y. Hirabayashi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

For understanding of the biological function of glycoconjugates during embryogenesis and morphogenesis, Xenopus laevis is considered a very useful animal model. We have found that blood-group-active molecules characteristically were distributed in the cell-cell contact region of Xenopus blastula cells. The chemical nature of blood-group-active glycoconjugates, including glycosphingolipids, is little known. T.l.c.-immunostaining using anti-blood-group-antigen antibodies showed that many species of blood group-B-active glycosphingolipids existed in the neutral glycosphingolipid fraction extracted from Xenopus laevis eggs. Among the B-active glycosphingolipids detected, two major components with the fastest mobility on a t.l.c. plate, tentatively termed XN-1 and XN-2, were isolated, and their chemical structures were characterized by gas chromatography-mass spectrometry, immunological analysis, fast-atom-bombardment mass spectrometry and 1H-n.m.r, spectroscopy. Both XN-1 and XN-2 had an identical pentaoligosaccharide structure, but differed in their ceramide moiety. The chemical structure is: Galα1-3 Galβ1-3Galβ1-4Glcβ1-1'Cer Fucα1-2 This is a novel type of pentaglycosylceramide with blood-group B activity, in that it lacks N-acetylhexosamine in its core carbohydrate structure, In this paper, a possible involvement of the blood-group antigen in the cell-adhesion process of Xenopus embryonic cells is discussed.

Original languageEnglish
Pages (from-to)821-827
Number of pages7
JournalBiochemical Journal
Volume306
Issue number3
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Blastula
Fucose
Cell adhesion
Blood Group Antigens
Xenopus
Cell Adhesion
Glycosphingolipids
Glycoconjugates
Xenopus laevis
Mass spectrometry
Neutral Glycosphingolipids
Fast Atom Bombardment Mass Spectrometry
Ceramides
Morphogenesis
Gas chromatography
Gas Chromatography-Mass Spectrometry
Eggs
Embryonic Development
pentaglycosyl ceramide
Spectrum Analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Occurrence of a novel fucose-containing pentaglycosylceramide with blood-group-B active determinant in Xenopus blastula cells : Its possible involvement in cell-cell adhesion. / Nomura, Kazuya; Nakajo, Nobushige; Hidari, K. L.P.J.; Nomura, H.; Murata, M.; Suzuki, M.; Yamana, K.; Hirabayashi, Y.

In: Biochemical Journal, Vol. 306, No. 3, 01.01.1995, p. 821-827.

Research output: Contribution to journalArticle

Nomura, Kazuya ; Nakajo, Nobushige ; Hidari, K. L.P.J. ; Nomura, H. ; Murata, M. ; Suzuki, M. ; Yamana, K. ; Hirabayashi, Y. / Occurrence of a novel fucose-containing pentaglycosylceramide with blood-group-B active determinant in Xenopus blastula cells : Its possible involvement in cell-cell adhesion. In: Biochemical Journal. 1995 ; Vol. 306, No. 3. pp. 821-827.
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