Abstract
For understanding of the biological function of glycoconjugates during embryogenesis and morphogenesis, Xenopus laevis is considered a very useful animal model. We have found that blood-group-active molecules characteristically were distributed in the cell-cell contact region of Xenopus blastula cells. The chemical nature of blood-group-active glycoconjugates, including glycosphingolipids, is little known. T.l.c.-immunostaining using anti-blood-group-antigen antibodies showed that many species of blood group-B-active glycosphingolipids existed in the neutral glycosphingolipid fraction extracted from Xenopus laevis eggs. Among the B-active glycosphingolipids detected, two major components with the fastest mobility on a t.l.c. plate, tentatively termed XN-1 and XN-2, were isolated, and their chemical structures were characterized by gas chromatography-mass spectrometry, immunological analysis, fast-atom-bombardment mass spectrometry and 1H-n.m.r, spectroscopy. Both XN-1 and XN-2 had an identical pentaoligosaccharide structure, but differed in their ceramide moiety. The chemical structure is: Galα1-3 Galβ1-3Galβ1-4Glcβ1-1'Cer Fucα1-2 This is a novel type of pentaglycosylceramide with blood-group B activity, in that it lacks N-acetylhexosamine in its core carbohydrate structure, In this paper, a possible involvement of the blood-group antigen in the cell-adhesion process of Xenopus embryonic cells is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 821-827 |
| Number of pages | 7 |
| Journal | Biochemical Journal |
| Volume | 306 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 1995 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Occurrence of a novel fucose-containing pentaglycosylceramide with blood-group-B active determinant in Xenopus blastula cells : Its possible involvement in cell-cell adhesion. / Nomura, Kazuya; Nakajo, Nobushige; Hidari, K. L.P.J.; Nomura, H.; Murata, M.; Suzuki, M.; Yamana, K.; Hirabayashi, Y.
In: Biochemical Journal, Vol. 306, No. 3, 01.01.1995, p. 821-827.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Occurrence of a novel fucose-containing pentaglycosylceramide with blood-group-B active determinant in Xenopus blastula cells
T2 - Its possible involvement in cell-cell adhesion
AU - Nomura, Kazuya
AU - Nakajo, Nobushige
AU - Hidari, K. L.P.J.
AU - Nomura, H.
AU - Murata, M.
AU - Suzuki, M.
AU - Yamana, K.
AU - Hirabayashi, Y.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - For understanding of the biological function of glycoconjugates during embryogenesis and morphogenesis, Xenopus laevis is considered a very useful animal model. We have found that blood-group-active molecules characteristically were distributed in the cell-cell contact region of Xenopus blastula cells. The chemical nature of blood-group-active glycoconjugates, including glycosphingolipids, is little known. T.l.c.-immunostaining using anti-blood-group-antigen antibodies showed that many species of blood group-B-active glycosphingolipids existed in the neutral glycosphingolipid fraction extracted from Xenopus laevis eggs. Among the B-active glycosphingolipids detected, two major components with the fastest mobility on a t.l.c. plate, tentatively termed XN-1 and XN-2, were isolated, and their chemical structures were characterized by gas chromatography-mass spectrometry, immunological analysis, fast-atom-bombardment mass spectrometry and 1H-n.m.r, spectroscopy. Both XN-1 and XN-2 had an identical pentaoligosaccharide structure, but differed in their ceramide moiety. The chemical structure is: Galα1-3 Galβ1-3Galβ1-4Glcβ1-1'Cer Fucα1-2 This is a novel type of pentaglycosylceramide with blood-group B activity, in that it lacks N-acetylhexosamine in its core carbohydrate structure, In this paper, a possible involvement of the blood-group antigen in the cell-adhesion process of Xenopus embryonic cells is discussed.
AB - For understanding of the biological function of glycoconjugates during embryogenesis and morphogenesis, Xenopus laevis is considered a very useful animal model. We have found that blood-group-active molecules characteristically were distributed in the cell-cell contact region of Xenopus blastula cells. The chemical nature of blood-group-active glycoconjugates, including glycosphingolipids, is little known. T.l.c.-immunostaining using anti-blood-group-antigen antibodies showed that many species of blood group-B-active glycosphingolipids existed in the neutral glycosphingolipid fraction extracted from Xenopus laevis eggs. Among the B-active glycosphingolipids detected, two major components with the fastest mobility on a t.l.c. plate, tentatively termed XN-1 and XN-2, were isolated, and their chemical structures were characterized by gas chromatography-mass spectrometry, immunological analysis, fast-atom-bombardment mass spectrometry and 1H-n.m.r, spectroscopy. Both XN-1 and XN-2 had an identical pentaoligosaccharide structure, but differed in their ceramide moiety. The chemical structure is: Galα1-3 Galβ1-3Galβ1-4Glcβ1-1'Cer Fucα1-2 This is a novel type of pentaglycosylceramide with blood-group B activity, in that it lacks N-acetylhexosamine in its core carbohydrate structure, In this paper, a possible involvement of the blood-group antigen in the cell-adhesion process of Xenopus embryonic cells is discussed.
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U2 - 10.1042/bj3060821
DO - 10.1042/bj3060821
M3 - Article
VL - 306
SP - 821
EP - 827
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
IS - 3
ER -