Occurrence of an allosteric transition in the modification of papain withl-1-acetyl-2,3-dihydropyrrolo[2,3-b]-indole-2-carboxamide

Akinori Nagatomo, Kohta Sakai, Takeru Nose, Yasuyuki Shimohigashi, Motonori Ohno

Research output: Contribution to journalArticle

Abstract

When papain was reacted with l-1-acetyl-2,3-dihydropyrrolo[2,3-b]indole-2-carboxamide at pH 8.0, inactivation occurred accompanied by modification of Cys-25 in the active site. Plots of pseudo first-order rate constants against the reagent concentrations yielded an anomalous sigmoidal curve, suggesting that papain responded to this reagent in an allosteric manner. This is supported by the fact that the presence of a moderate concentration (a twenty-fold molar excess) of Nα-acetyl-l-tryptophanamide over papain accelerated the inactivation.

Original languageEnglish
Pages (from-to)411-413
Number of pages3
JournalJournal of Chromatography A
Volume597
Issue number1-2
DOIs
Publication statusPublished - Apr 24 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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