Occurrence of Keratan Sulfate in Connective Tissues of Teleost

Makoto Ito, Tetuo Morinaga, Manabu Kitamikado

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new method for the detection of keratan sulfate in connective tissue has been developed without its prior isolation from a mixture of glycosaminoglycan. The method relies on the specific action of an endo-β-galactosidase from Flavobacterium sp. on keratan sulfate. Besides being found in mammals, keratan sulfate is widely distributed in the connective tissues of teleost; it is especially abundant in the skin, cornea, and caudal fin of Pacific mackerel, and in the cornea of horse mackerel. From the skin of Pacific mackerel, a crude preparation of polysaccharide was obtained, which contained a large amount of carbohydrate chains susceptible to endo-β-galactosidase. The fraction with the carbohydrate chains was isolated by treating the preparation with hyaluronidase and chondroitinase ABC. The purified substance was characterized using chemical and infrared spectrum analyses, and was identified as keratan sulfate. This paper is the first report to demonstrate the presence of keratan sulfate in skin tissue.

Original languageEnglish
Pages (from-to)1445-1452
Number of pages8
JournalNIPPON SUISAN GAKKAISHI
Volume48
Issue number10
DOIs
Publication statusPublished - Jan 1 1982

Fingerprint

teleost
connective tissues
sulfates
sulfate
mackerel
skin (animal)
galactosidases
skin
cornea
carbohydrate
carbohydrates
hyaluronoglucosaminidase
Flavobacterium
glycosaminoglycans
horse
polysaccharide
fins
mammal
polysaccharides
tissue

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Occurrence of Keratan Sulfate in Connective Tissues of Teleost. / Ito, Makoto; Morinaga, Tetuo; Kitamikado, Manabu.

In: NIPPON SUISAN GAKKAISHI, Vol. 48, No. 10, 01.01.1982, p. 1445-1452.

Research output: Contribution to journalArticle

Ito, Makoto ; Morinaga, Tetuo ; Kitamikado, Manabu. / Occurrence of Keratan Sulfate in Connective Tissues of Teleost. In: NIPPON SUISAN GAKKAISHI. 1982 ; Vol. 48, No. 10. pp. 1445-1452.
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