Octacalcium phosphate-precipitated alginate scaffold for bone regeneration

Takeshi Fuji, Takahisa Anada, Yoshitomo Honda, Yukari Shiwaku, Hiroko Koike, Shinji Kamakura, Keiichi Sasaki, Osamu Suzuki

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The present study was designed to investigate whether octacalcium phosphate (OCP)-precipitated alginate (Alg) promotes osteoblastic cell proliferation and bone regeneration in comparison with Alg itself. Alg, known to lack mammal cell attachment capability, was used as the matrix to test the distribution effect of OCP in a three-dimensional environment. A series of Alg/OCP scaffolds with different pore sizes was prepared by centrifuging Alg gels precipitated by OCP crystals. The scaffolds had a bimodal distribution of pores (ultrafine pores: approximately 100nm; relatively large pores: from 6.0 to 51.7 μm) and over 86% porosity. The osteoconductive capability of Alg/OCP was determined by examining mouse bone marrow stromal ST-2 cell proliferation after 3 days in vitro and bone regeneration in mouse calvaria critical-sized defect after 21 days. The analyses showed that ST-2 cell proliferation and bone regeneration increased with an increase in the pore size and reached the highest level in the 51.7 μm pore scaffold. The results suggest that OCP-precipitated Alg provides a better scaffold for osteoblasts to attach and proliferate in a three-dimensional environment and promotes bone regeneration, indicating that OCP is a candidate material to modify the surface of non-cell-interactive polymeric scaffolds, such as Alg, into an osteogenic condition.

Original languageEnglish
Pages (from-to)3525-3535
Number of pages11
JournalTissue Engineering - Part A
Volume15
Issue number11
DOIs
Publication statusPublished - Nov 1 2009
Externally publishedYes

Fingerprint

Bone Regeneration
Alginate
Scaffolds
Bone
Phosphates
Cell proliferation
Cell Proliferation
Pore size
Mammals
alginic acid
octacalcium phosphate
Porosity
Osteoblasts
Skull
Gels
Bone Marrow
Defects
Crystals

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Fuji, T., Anada, T., Honda, Y., Shiwaku, Y., Koike, H., Kamakura, S., ... Suzuki, O. (2009). Octacalcium phosphate-precipitated alginate scaffold for bone regeneration. Tissue Engineering - Part A, 15(11), 3525-3535. https://doi.org/10.1089/ten.tea.2009.0048

Octacalcium phosphate-precipitated alginate scaffold for bone regeneration. / Fuji, Takeshi; Anada, Takahisa; Honda, Yoshitomo; Shiwaku, Yukari; Koike, Hiroko; Kamakura, Shinji; Sasaki, Keiichi; Suzuki, Osamu.

In: Tissue Engineering - Part A, Vol. 15, No. 11, 01.11.2009, p. 3525-3535.

Research output: Contribution to journalArticle

Fuji, T, Anada, T, Honda, Y, Shiwaku, Y, Koike, H, Kamakura, S, Sasaki, K & Suzuki, O 2009, 'Octacalcium phosphate-precipitated alginate scaffold for bone regeneration', Tissue Engineering - Part A, vol. 15, no. 11, pp. 3525-3535. https://doi.org/10.1089/ten.tea.2009.0048
Fuji, Takeshi ; Anada, Takahisa ; Honda, Yoshitomo ; Shiwaku, Yukari ; Koike, Hiroko ; Kamakura, Shinji ; Sasaki, Keiichi ; Suzuki, Osamu. / Octacalcium phosphate-precipitated alginate scaffold for bone regeneration. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 11. pp. 3525-3535.
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