Octadecylsilyl silica column method for extraction of messenger RNA

Taro Kimura, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have developed a simple and rapid method for the purification of poly(A) tail-messenger RNA (mRNA) from total RNA by using a solid phase extraction column filled with a small amount of octadecylsilyl silica. The method is based on a hydrophobic interaction between the poly(A) tail and the octadecyl unit on the silica particle in a water/dimethyl sulfoxide mixed solution. By using this column, mRNA can be separated from 100 μg of total RNA in less than 10 min with high yields (>80%).

Original languageEnglish
Pages (from-to)72-73
Number of pages2
JournalAnalytical Biochemistry
Volume391
Issue number1
DOIs
Publication statusPublished - Aug 1 2009
Externally publishedYes

Fingerprint

Messenger RNA
RNA
Solid Phase Extraction
Dimethyl Sulfoxide
Hydrophobic and Hydrophilic Interactions
Silicon Dioxide
Purification
octadecylsilica
Water

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Octadecylsilyl silica column method for extraction of messenger RNA. / Kimura, Taro; Sakurai, Kazuo; Shinkai, Seiji.

In: Analytical Biochemistry, Vol. 391, No. 1, 01.08.2009, p. 72-73.

Research output: Contribution to journalArticle

Kimura, Taro ; Sakurai, Kazuo ; Shinkai, Seiji. / Octadecylsilyl silica column method for extraction of messenger RNA. In: Analytical Biochemistry. 2009 ; Vol. 391, No. 1. pp. 72-73.
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