Ocular immune privilege and CD1d-reactive natural killer T cells

Kohei Sonoda, Joan Stein-Streilein

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Purpose. Immune privilege in the eye is, in part, associated with the development of an antigen-specific systemic tolerance termed anterior chamber-associated immune deviation (ACAID). Natural killer T (NKT) cells express T-cell receptor and natural killer (NK) markers and are classified as innate immune cells partly because they produce cytokines within minutes of signals. The aim of this study was to elucidate the role of murine NKT cells in the induction of T regulatory cells in anterior chamber-associated immune deviation. Methods. Anterior chamber-associated immune deviation T regulatory cell generation ability was examined in the following NKT cell-deficient mice: SJL mice, CD1d or Jα281 knockout (KO) mice on C57BL/6 (B6) background, and NKT cell-depleted mice. To detect T regulatory cells, splenic T cells were harvested 7 days after anterior chamber inoculation of ovalbumin (50 μg/2 μL in Hanks balanced salt solution [HBSS]), mixed with ovalbumin-primed T cells (effector) and ovalbumin-pulsed antigen-presenting cells (stimulator), and then cotransferred into the ear pinnae of a syngeneic naive mouse (local adoptive transfer assay). Ear swelling was measured 24 hours later. Results. Anterior chamber-inoculated B6 mice developed T regulatory cells, but all natural killer T cell-deficient mice did not generate T regulatory cells unless they were reconstituted with natural killer T cells. We also found that the number of splenic natural killer T cells were increased in anterior chamber-inoculated B6 mice and those natural killer T cells produced IL-10. Conclusions. CDld-reactive natural killer T cells are essential for the induction of T regulatory cells in anterior chamber-associated immune deviation through their IL-10 production and are involved in the maintenance of immune privilege of the eye.

Original languageEnglish
JournalCornea
Volume21
Issue number2 SUPPL. 1
DOIs
Publication statusPublished - 2002
Externally publishedYes

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Natural Killer T-Cells
Anterior Chamber
Regulatory T-Lymphocytes
Ovalbumin
Interleukin-10
Ear Auricle
T-Lymphocytes
Adoptive Transfer
Antigen-Presenting Cells
T-Cell Antigen Receptor
Knockout Mice
Ear
Maintenance
Cytokines
Antigens

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Ocular immune privilege and CD1d-reactive natural killer T cells. / Sonoda, Kohei; Stein-Streilein, Joan.

In: Cornea, Vol. 21, No. 2 SUPPL. 1, 2002.

Research output: Contribution to journalArticle

Sonoda, Kohei ; Stein-Streilein, Joan. / Ocular immune privilege and CD1d-reactive natural killer T cells. In: Cornea. 2002 ; Vol. 21, No. 2 SUPPL. 1.
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AB - Purpose. Immune privilege in the eye is, in part, associated with the development of an antigen-specific systemic tolerance termed anterior chamber-associated immune deviation (ACAID). Natural killer T (NKT) cells express T-cell receptor and natural killer (NK) markers and are classified as innate immune cells partly because they produce cytokines within minutes of signals. The aim of this study was to elucidate the role of murine NKT cells in the induction of T regulatory cells in anterior chamber-associated immune deviation. Methods. Anterior chamber-associated immune deviation T regulatory cell generation ability was examined in the following NKT cell-deficient mice: SJL mice, CD1d or Jα281 knockout (KO) mice on C57BL/6 (B6) background, and NKT cell-depleted mice. To detect T regulatory cells, splenic T cells were harvested 7 days after anterior chamber inoculation of ovalbumin (50 μg/2 μL in Hanks balanced salt solution [HBSS]), mixed with ovalbumin-primed T cells (effector) and ovalbumin-pulsed antigen-presenting cells (stimulator), and then cotransferred into the ear pinnae of a syngeneic naive mouse (local adoptive transfer assay). Ear swelling was measured 24 hours later. Results. Anterior chamber-inoculated B6 mice developed T regulatory cells, but all natural killer T cell-deficient mice did not generate T regulatory cells unless they were reconstituted with natural killer T cells. We also found that the number of splenic natural killer T cells were increased in anterior chamber-inoculated B6 mice and those natural killer T cells produced IL-10. Conclusions. CDld-reactive natural killer T cells are essential for the induction of T regulatory cells in anterior chamber-associated immune deviation through their IL-10 production and are involved in the maintenance of immune privilege of the eye.

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