Odorant response assays for a heterologously expressed olfactory receptor

Sayako Katada, Takao Nakagawa, Hiroshi Kataoka, Kazushige Touhara

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

Odorant responsiveness of a mouse olfactory receptor, mOR-EG, was investigated in various heterologous cells using a variety of detection methods. Odorant-induced Ca2+ response was observed in HEK293 cells that coexpressed mOR-EG and the promiscuous G protein, Gα15. Without Gα15, a robust increase in cAMP level was observed upon odorant-stimulation in various mammalian cells. A luciferase reporter gene assay using zif268 promoter was adopted to amplify the cAMP signals. In Xenopus laevis oocytes, odorant-stimulated currents were recorded when mOR-EG cRNA was co-injected with either Gα15 or cAMP-dependent channel. These results suggest that odorant responsiveness can be monitored via a signaling pathway mediated by endogenous Gαs or transfected Gα15 in heterologous cell systems. Various functional assays for a heterologously expressed olfactory receptor reported in this study, are potentially useful for high-throughput ligand screening and functional analyses of hundreds of olfactory receptors.

Original languageEnglish
Pages (from-to)964-969
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume305
Issue number4
DOIs
Publication statusPublished - Jun 13 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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