Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1

Hitoshi Inada, Fuminori Kawabata, Yoshiro Ishimaru, Tohru Fushiki, Hiroaki Matsunami, Makoto Tominaga

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Ligand-gated ion channels are important in sensory and synaptic transduction. The PKD1L3-PKD2L1 channel complex is a sour taste receptor candidate that is activated by acids. Here, we report that the proton-activated PKD1L3-PKD2L1 ion channels have the unique ability to be activated after the removal of an acid stimulus. We refer to this property as the off-response (previously described as a delayed response). Electrophysiological analyses show that acid-induced responses are observed only after the removal of an acid solution at less than pH 3.0. A small increase in pH is sufficient for PKD1L3-PKD2L1 channel activation, after exposure to an acid at pH 2.5. These results indicate that this channel is a new type of ion channel-designated as an 'off-channel'-which is activated during stimulus application but not gated open until the removal of the stimulus. The off-response property of PKD1L3-PKD2L1 channels might explain the physiological phenomena occurring during sour taste sensation.

Original languageEnglish
Pages (from-to)690-697
Number of pages8
JournalEMBO Reports
Volume9
Issue number7
DOIs
Publication statusPublished - Jul 1 2008

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Cations
Acids
Ion Channels
Physiological Phenomena
Ligand-Gated Ion Channels
Aptitude
Protons
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Inada, H., Kawabata, F., Ishimaru, Y., Fushiki, T., Matsunami, H., & Tominaga, M. (2008). Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1. EMBO Reports, 9(7), 690-697. https://doi.org/10.1038/embor.2008.89

Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1. / Inada, Hitoshi; Kawabata, Fuminori; Ishimaru, Yoshiro; Fushiki, Tohru; Matsunami, Hiroaki; Tominaga, Makoto.

In: EMBO Reports, Vol. 9, No. 7, 01.07.2008, p. 690-697.

Research output: Contribution to journalArticle

Inada, H, Kawabata, F, Ishimaru, Y, Fushiki, T, Matsunami, H & Tominaga, M 2008, 'Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1', EMBO Reports, vol. 9, no. 7, pp. 690-697. https://doi.org/10.1038/embor.2008.89
Inada H, Kawabata F, Ishimaru Y, Fushiki T, Matsunami H, Tominaga M. Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1. EMBO Reports. 2008 Jul 1;9(7):690-697. https://doi.org/10.1038/embor.2008.89
Inada, Hitoshi ; Kawabata, Fuminori ; Ishimaru, Yoshiro ; Fushiki, Tohru ; Matsunami, Hiroaki ; Tominaga, Makoto. / Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1. In: EMBO Reports. 2008 ; Vol. 9, No. 7. pp. 690-697.
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