Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice

Katsuhiko Hayashi, Sugako Ogushi, Kazuki Kurimoto, So Shimamoto, Hiroshi Ohta, Mitinori Saitou

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385 Citations (Scopus)

Abstract

Reconstitution of female germ cell development in vitro is a key challenge in reproductive biology and medicine. We show here that female (XX) embryonic stem cells and induced pluripotent stem cells in mice are induced into primordial germ cell-like cells (PGCLCs), which, when aggregated with female gonadal somatic cells as reconstituted ovaries, undergo X-reactivation, imprint erasure, and cyst formation, and exhibit meiotic potential. Upon transplantation under mouse ovarian bursa, PGCLCs in the reconstituted ovaries mature into germinal vesicle-stage oocytes, which then contribute to fertile offspring after in vitro maturation and fertilization. Our culture system serves as a robust foundation for the investigation of key properties of female germ cells, including the acquisition of totipotency, and for the reconstitution of whole female germ cell development in vitro.

Original languageEnglish
Pages (from-to)971-975
Number of pages5
JournalScience
Volume338
Issue number6109
DOIs
Publication statusPublished - Nov 16 2012

All Science Journal Classification (ASJC) codes

  • General

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    Hayashi, K., Ogushi, S., Kurimoto, K., Shimamoto, S., Ohta, H., & Saitou, M. (2012). Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice. Science, 338(6109), 971-975. https://doi.org/10.1126/science.1226889