OIP5-AS1 promotes proliferation of non-small-cell lung cancer and head and neck squamous cell carcinoma cells

Yojiro Kotake, Natsumi Matsunaga, Takahiro Wakasaki, Ryotaro Okada

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aim: The long noncoding RNA OIP5 antisense RNA 1 (OIP5-AS1) is overexpressed in various cancer types, such as lung cancer, hepatoblastoma and cervical cancer, and functions to accelerate cell proliferation, invasion and migration. Here, we investigated the roIe of OIP5-AS1 in cell-cycle progression of H1299 and A549 non-small cell lung cancer cells, and FaDu and CAL27 head and neck squamous cell carcinoma cells. Materials and Methods: The cells were transfected with small interfering RNA and subjected to cell-cycle analysis and reverse-transcription quantitative polymerase chain reaction (RT-qPCR). Results: Silencing of OIP5-AS1 suppressed the proliferation of H1299, A549, FaDu and CAL27 cells. RT-qPCR and cell-cycle analysis revealed that silencing OIP5-AS1 increased the expression of CDK inhibitors, such as p15, p16, p18 and p19, resulting in G1-phase arrest. Conclusion: OIP5-AS1 regulates G1-phase progression by repressing CDK inhibitors and, thus, promotes the proliferation of H1299, A549, FaDu and CAL27 cells.

Original languageEnglish
Pages (from-to)543-548
Number of pages6
JournalCancer Genomics and Proteomics
Volume18
Issue number4
DOIs
Publication statusPublished - Jul 2021

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint

Dive into the research topics of 'OIP5-AS1 promotes proliferation of non-small-cell lung cancer and head and neck squamous cell carcinoma cells'. Together they form a unique fingerprint.

Cite this