Oleic acid up-regulates myosin heavy chain (MyHC) 1 expression and increases mitochondrial mass and maximum respiration in C2C12 myoblasts

Naomi Watanabe, Yusuke Komiya, Yusuke Sato, Yusuke Watanabe, Takahiro Suzuki, Keizo Arihara

Research output: Contribution to journalArticlepeer-review

Abstract

Skeletal muscle is divided into type 1 and type 2 fibers. Type 1 fibers are rich in mitochondria, have high oxidative metabolism, and are resistant to fatigue. Muscle-specific overexpression of peroxisome proliferator-activated receptor (PPAR)δ drastically increases the number of type 1 fibers. We focused on oleic acid, an omega-9 monounsaturated fatty acid, as a factor that activates PPARδ. In this study, we examined the effects of oleic acid on the muscle fiber type of C2C12 myotubes and its relationship with PPARδ. Our results showed that oleic acid treatment increased the levels of myosin heavy chain (MyHC)1, a known type 1 fiber marker, as well as mitochondrial mass and maximum respiration in C2C12 cells. To confirm the relationship between PPARδ activation and oleic acid-induced MyHC1 increase, we examined the effects of oleic acid in PPARδ knockdown C2C12 myoblasts. We found that oleic acid supplementation increased the mRNA expression of MyHC1 in PPARδ-knockdown C2C12 cells. Our data suggest that oleic acid increases type 1 fiber levels in C2C12 myotubes in a PPARδ-independent manner.

Original languageEnglish
Pages (from-to)406-411
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume525
Issue number2
DOIs
Publication statusPublished - Apr 30 2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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