Oligoclonal T lymphocytes infiltrating human lung cancer tissues

Ichiro Yoshino, Tokujiro Yano, Yasunobu Yoshikai, Mitsuhiro Murata, Keizo Sugimachi, Genki Kimura, Kikuo Nomoto

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    To clarify the nature of tumor‐infiltrating lymphocytes (TILs), we investigated the possible clonality of the T cells in TILs freshly isolated from human primary lung cancer tissues by assessing the rearrangement pattern of the T‐cell receptor (TCR) gene P locus using Southern blotting. First, in phenotypic analysis, TILs represented different populations among corresponding peripheral blood lymphocytes (PBLs) with an increased proportion of CD20+ (B) cells as well as a decreased proportion of CD 16+ (natural killer) cells, and a variable CD4/ CD8 ratio. Considering the central role of T cells in immune responses, we analyzed TCR β gene rearrangement patterns in TILs and corresponding PBLs from 12 patients. In 10 of the 12 cases, TILs showed one or more TCR gene rearrangement bands with a predominance of the C.β2 gene, in which 2 types of common rearranged band were observed among the cases with different clinical profiles in terms of histological types and disease stage, with bands at about 9.5 kb in 7 and at 11.5 kb in 8 patients. On the other hand, predominant rearranged bands were hardly detected in corresponding PBLs except in 2 cases. From these results, we conclude that TILs in lung cancer tissues frequently contain oligoclonal T‐cell populations, which were probably sensitized by relatively common antigens at the tumor sites.

    Original languageEnglish
    Pages (from-to)654-658
    Number of pages5
    JournalInternational Journal of Cancer
    Volume47
    Issue number5
    DOIs
    Publication statusPublished - Jan 1 1991

    Fingerprint

    Lung Neoplasms
    Lymphocytes
    T-Lymphocytes
    Gene Rearrangement
    CD4-CD8 Ratio
    Neoplasm Antigens
    Southern Blotting
    Natural Killer Cells
    Population
    Genes
    B-Lymphocytes

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

    Cite this

    Yoshino, I., Yano, T., Yoshikai, Y., Murata, M., Sugimachi, K., Kimura, G., & Nomoto, K. (1991). Oligoclonal T lymphocytes infiltrating human lung cancer tissues. International Journal of Cancer, 47(5), 654-658. https://doi.org/10.1002/ijc.2910470504

    Oligoclonal T lymphocytes infiltrating human lung cancer tissues. / Yoshino, Ichiro; Yano, Tokujiro; Yoshikai, Yasunobu; Murata, Mitsuhiro; Sugimachi, Keizo; Kimura, Genki; Nomoto, Kikuo.

    In: International Journal of Cancer, Vol. 47, No. 5, 01.01.1991, p. 654-658.

    Research output: Contribution to journalArticle

    Yoshino, I, Yano, T, Yoshikai, Y, Murata, M, Sugimachi, K, Kimura, G & Nomoto, K 1991, 'Oligoclonal T lymphocytes infiltrating human lung cancer tissues', International Journal of Cancer, vol. 47, no. 5, pp. 654-658. https://doi.org/10.1002/ijc.2910470504
    Yoshino I, Yano T, Yoshikai Y, Murata M, Sugimachi K, Kimura G et al. Oligoclonal T lymphocytes infiltrating human lung cancer tissues. International Journal of Cancer. 1991 Jan 1;47(5):654-658. https://doi.org/10.1002/ijc.2910470504
    Yoshino, Ichiro ; Yano, Tokujiro ; Yoshikai, Yasunobu ; Murata, Mitsuhiro ; Sugimachi, Keizo ; Kimura, Genki ; Nomoto, Kikuo. / Oligoclonal T lymphocytes infiltrating human lung cancer tissues. In: International Journal of Cancer. 1991 ; Vol. 47, No. 5. pp. 654-658.
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