Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules

Ryusei Tsuruga, Narumi Uehara, Yuki Suzuki, Hiroyuki Furuta, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.

Original languageEnglish
Pages (from-to)410-415
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume128
Issue number4
DOIs
Publication statusPublished - Oct 2019

Fingerprint

Catalytic RNA
Oligomerization
Oligomers
RNA
Tetrahymena
Dissection
Nanostructures
Atomic Force Microscopy
Atomic force microscopy

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules. / Tsuruga, Ryusei; Uehara, Narumi; Suzuki, Yuki; Furuta, Hiroyuki; Sugiyama, Hiroshi; Endo, Masayuki; Matsumura, Shigeyoshi; Ikawa, Yoshiya.

In: Journal of Bioscience and Bioengineering, Vol. 128, No. 4, 10.2019, p. 410-415.

Research output: Contribution to journalArticle

Tsuruga, Ryusei ; Uehara, Narumi ; Suzuki, Yuki ; Furuta, Hiroyuki ; Sugiyama, Hiroshi ; Endo, Masayuki ; Matsumura, Shigeyoshi ; Ikawa, Yoshiya. / Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules. In: Journal of Bioscience and Bioengineering. 2019 ; Vol. 128, No. 4. pp. 410-415.
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