On archaeal homologs of the human RNase P proteins Pop5 and Rpp30 in the hyperthermophilic archaeon Thermococcus kodakarensis

Kotaro Suematsu, Toshifumi Ueda, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ribonuclease P (RNase P) proteins TkoPop5 and TkoRpp30, homologs of human Pop5 and Rpp30, respectively, in the hyperthermophilic archa-eon Thermococcus kodakarensis were prepared and characterized with respect to pre-tRNA cleavage activity using the reconstitution system of the well-studied Pyrococcus horikoshii RNase P. The reconstituted particle containing TkoPop5 in place of the P. horikoshii counterpart PhoPop5 retained pre-tRNA cleavage activity comparable to that of the reconstituted P. horikoshii RNase P, while that containing TkoRpp30 instead of its corresponding protein PhoRpp30 had slightly lower activity than the P. horikoshii RNase P. Moreover, we determined crystal structures of TkoRpp30 alone and in complex with TkoPop5. Like their P. horikoshii counterparts, whose structures were solved previously, TkoRpp30 and TkoPop5 fold into TIM barrel and RRM-like fold, respectively. This finding demonstrates that RNase P proteins in T. kodakarensis and P. horiko-shii are interchangeable and that their three-dimensional structures are highly conserved.

Original languageEnglish
Pages (from-to)952-959
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number6
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Pyrococcus horikoshii
Thermococcus
Ribonuclease P
Archaea
RNA Precursors
Proteins
Crystal structure
human OCA2 protein

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

On archaeal homologs of the human RNase P proteins Pop5 and Rpp30 in the hyperthermophilic archaeon Thermococcus kodakarensis. / Suematsu, Kotaro; Ueda, Toshifumi; Nakashima, Takashi; Kakuta, Yoshimitsu; Kimura, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 79, No. 6, 01.01.2015, p. 952-959.

Research output: Contribution to journalArticle

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AU - Kakuta, Yoshimitsu

AU - Kimura, Makoto

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