TY - JOUR
T1 - On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge
AU - Yamanishi, Yoko
AU - Teramoto, Jun
AU - Magariyama, Yukio
AU - Ishihama, Akira
AU - Fukuda, Toshio
AU - Arai, Fumihito
N1 - Funding Information:
Manuscript received April 22, 2009. First published October 30, 2009; current version published February 3, 2010. This work was supported by the Ministry of Education, Culture, Sports, Science and Technology under Grants-in-Aid for Scientific Research (20034003). Asterisk indicates corresponding author.
PY - 2009/12
Y1 - 2009/12
N2 - In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.
AB - In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.
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U2 - 10.1109/TNB.2009.2035273
DO - 10.1109/TNB.2009.2035273
M3 - Article
C2 - 19884104
AN - SCOPUS:76949106682
VL - 8
SP - 312
EP - 317
JO - IEEE Transactions on Nanobioscience
JF - IEEE Transactions on Nanobioscience
SN - 1536-1241
IS - 4
M1 - 5299057
ER -