Abstract
In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.
| Original language | English |
|---|---|
| Article number | 5299057 |
| Pages (from-to) | 312-317 |
| Number of pages | 6 |
| Journal | IEEE Transactions on Nanobioscience |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 1 2009 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Medicine (miscellaneous)
- Biomedical Engineering
- Pharmaceutical Science
- Computer Science Applications
- Electrical and Electronic Engineering
Cite this
On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge. / Yamanishi, Yoko; Teramoto, Jun; Magariyama, Yukio; Ishihama, Akira; Fukuda, Toshio; Arai, Fumihito.
In: IEEE Transactions on Nanobioscience, Vol. 8, No. 4, 5299057, 01.12.2009, p. 312-317.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge
AU - Yamanishi, Yoko
AU - Teramoto, Jun
AU - Magariyama, Yukio
AU - Ishihama, Akira
AU - Fukuda, Toshio
AU - Arai, Fumihito
PY - 2009/12/1
Y1 - 2009/12/1
N2 - In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.
AB - In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.
UR - http://www.scopus.com/inward/record.url?scp=76949106682&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76949106682&partnerID=8YFLogxK
U2 - 10.1109/TNB.2009.2035273
DO - 10.1109/TNB.2009.2035273
M3 - Article
C2 - 19884104
AN - SCOPUS:76949106682
VL - 8
SP - 312
EP - 317
JO - IEEE Transactions on Nanobioscience
JF - IEEE Transactions on Nanobioscience
SN - 1536-1241
IS - 4
M1 - 5299057
ER -