On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge

Yoko Yamanishi, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

Original languageEnglish
Article number5299057
Pages (from-to)312-317
Number of pages6
JournalIEEE Transactions on Nanobioscience
Volume8
Issue number4
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Fingerprint

Cell immobilization
Immobilization
Gels
Yeast
Monitoring
Yeasts
Micromachining
Gelation
Microtechnology
Microchannels
Tin oxides
Microfluidics
Hydrogels
Indium
Hydrogel
Dyes
Fluorescent Dyes
Cells
Fabrication
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge. / Yamanishi, Yoko; Teramoto, Jun; Magariyama, Yukio; Ishihama, Akira; Fukuda, Toshio; Arai, Fumihito.

In: IEEE Transactions on Nanobioscience, Vol. 8, No. 4, 5299057, 01.12.2009, p. 312-317.

Research output: Contribution to journalArticle

Yamanishi, Yoko ; Teramoto, Jun ; Magariyama, Yukio ; Ishihama, Akira ; Fukuda, Toshio ; Arai, Fumihito. / On-chip cell immobilization and monitoring system using thermosensitive gel controlled by suspended polymeric microbridge. In: IEEE Transactions on Nanobioscience. 2009 ; Vol. 8, No. 4. pp. 312-317.
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