On-chip fabrication of mutifunctional envelope-type nanodevices for gene delivery

Hiroshi Kuramoto, Yeon Su Park, Noritada Kaji, Manabu Tokeshi, Kentaro Kogure, Yasuo Shinohara, Hideyoshi Harashima, Yoshinobu Baba

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Microfluidic devices may be highly beneficial to the rapid fabrication of small quantities of various nonviral vectors with different functionalities, which is indispensable for effective order-made gene therapy. We adapted a microfluidic chip-based approach for fabricating small quantities of nonviral vectors in a short time in preparation for order-made gene therapy applications. This approach permitted us to fabricate multifunctional envelope-type nanodevices (MENDs), composed of a compacted (or condensed) DNA core and a lipid bilayer membrane shell, which are considered as promising nonviral vectors for gene therapy applications. The on-chip fabrication of the MEND was very simple, rapid, convenient, and cost-effective compared with conventional methods. The size of the MEND showed strong dependence on the concentration and flow rate of the reaction precursors and could be controlled to be much smaller than that achievable by conventional methods. This, together with abovementioned merits, makes our microfluidic chip-based approach very attractive for the fabrication of MENDs for effective application to order-made gene therapy.

Original languageEnglish
Pages (from-to)2729-2733
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Volume391
Issue number8
DOIs
Publication statusPublished - Aug 1 2008

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Gene therapy
Genetic Therapy
Genes
Microfluidics
Fabrication
Lab-On-A-Chip Devices
Lipid bilayers
Lipid Bilayers
Membrane Lipids
Flow rate
Costs and Cost Analysis
Membranes
DNA
Costs

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

On-chip fabrication of mutifunctional envelope-type nanodevices for gene delivery. / Kuramoto, Hiroshi; Park, Yeon Su; Kaji, Noritada; Tokeshi, Manabu; Kogure, Kentaro; Shinohara, Yasuo; Harashima, Hideyoshi; Baba, Yoshinobu.

In: Analytical and Bioanalytical Chemistry, Vol. 391, No. 8, 01.08.2008, p. 2729-2733.

Research output: Contribution to journalArticle

Kuramoto, H, Park, YS, Kaji, N, Tokeshi, M, Kogure, K, Shinohara, Y, Harashima, H & Baba, Y 2008, 'On-chip fabrication of mutifunctional envelope-type nanodevices for gene delivery', Analytical and Bioanalytical Chemistry, vol. 391, no. 8, pp. 2729-2733. https://doi.org/10.1007/s00216-008-2124-7
Kuramoto, Hiroshi ; Park, Yeon Su ; Kaji, Noritada ; Tokeshi, Manabu ; Kogure, Kentaro ; Shinohara, Yasuo ; Harashima, Hideyoshi ; Baba, Yoshinobu. / On-chip fabrication of mutifunctional envelope-type nanodevices for gene delivery. In: Analytical and Bioanalytical Chemistry. 2008 ; Vol. 391, No. 8. pp. 2729-2733.
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