On-chip platelet production using three dimensional microchannel

H. Kumon, S. Sakuma, S. Nakamura, K. Eto, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We succeeded in on-chip platelet production using a bioreactor with a curve-shaped 3D microchannel. In order to produce platelets from megakaryocytes (MKs) in a microfluidic chip, it is required to trap MKs and to apply fluid force to it. Since MKs have a relatively big distribution in their size, it is difficult to effectively trap MKs by using conventional microfluidic chips having uniformly patterned pillars with discrete pitch size. Thus, we proposed a curve-shaped 3D microchannel whose height gradually decreases along the flow pass to trap MKs of various size. We fabricated the curve-shaped 3D microchannel by using grey-scale lithography and deep reactive ion etching (DRIE) techniques. Since our microfluidic chip was packaged by a glass substrate, we can observe the processes of platelet production with a time-resolved technique. Through the experiments of on-chip platelet production using MKs induced from human induced pluripotent stem cells (hiPSCs), we successfully trapped the MKs of various size corresponding to the channel height. The trapped MKs were exposed to fluid force in the microchannel, and resulted in producing platelets.

Original languageEnglish
Title of host publication2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages121-124
Number of pages4
ISBN (Electronic)9781538647820
DOIs
Publication statusPublished - Apr 24 2018
Externally publishedYes
Event31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom
Duration: Jan 21 2018Jan 25 2018

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2018-January
ISSN (Print)1084-6999

Conference

Conference31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
CountryUnited Kingdom
CityBelfast
Period1/21/181/25/18

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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