Abstract
A micro-chip based real-time PCR device has been developed for biorobustness research and systems biology. The device is constructed of a silicon-glass chip, a Peltier element and a microscopy. With the device, amplification of target gene on a plasmid and housekeeping gene were detected simultaneously, and the copy number of the plasmid could be estimated. We had optimized the device, and achieved detection of fluorescent intensity increase in reaction channels.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 627-629 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806421 |
| Publication status | Published - Jan 1 2009 |
| Event | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: Nov 1 2009 → Nov 5 2009 |
Publication series
| Name | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Other
| Other | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 |
|---|---|
| Country | Korea, Republic of |
| City | Jeju |
| Period | 11/1/09 → 11/5/09 |
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All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
On-chip real-time PCR for genetic tug-of-war (g-TOW) experiment. / Naito, Toyohiro; Yatsuhashi, Ai; Kaji, Noritada; Ando, Taeko; Sato, Kazuo; Moriya, Hisao; Kitano, Hiroaki; Okamoto, Yukihiro; Tokeshi, Manabu; Baba, Yosinobu.
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 627-629 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - On-chip real-time PCR for genetic tug-of-war (g-TOW) experiment
AU - Naito, Toyohiro
AU - Yatsuhashi, Ai
AU - Kaji, Noritada
AU - Ando, Taeko
AU - Sato, Kazuo
AU - Moriya, Hisao
AU - Kitano, Hiroaki
AU - Okamoto, Yukihiro
AU - Tokeshi, Manabu
AU - Baba, Yosinobu
PY - 2009/1/1
Y1 - 2009/1/1
N2 - A micro-chip based real-time PCR device has been developed for biorobustness research and systems biology. The device is constructed of a silicon-glass chip, a Peltier element and a microscopy. With the device, amplification of target gene on a plasmid and housekeeping gene were detected simultaneously, and the copy number of the plasmid could be estimated. We had optimized the device, and achieved detection of fluorescent intensity increase in reaction channels.
AB - A micro-chip based real-time PCR device has been developed for biorobustness research and systems biology. The device is constructed of a silicon-glass chip, a Peltier element and a microscopy. With the device, amplification of target gene on a plasmid and housekeeping gene were detected simultaneously, and the copy number of the plasmid could be estimated. We had optimized the device, and achieved detection of fluorescent intensity increase in reaction channels.
UR - http://www.scopus.com/inward/record.url?scp=84901770304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901770304&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901770304
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 627
EP - 629
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -