On-chip temperature control under suspended microbridge for cell immobilization and culture system

Yoko Yamanishi, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1683-1685
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Fingerprint

Cell immobilization
Cell culture
Temperature control
Gels
Yeast
Micromachining
Gelation
Microchannels
Tin oxides
Microfluidics
Hydrogels
Indium
Hydrogel
Dyes
Fluorescent Dyes
Cells
Fabrication
poly-N-isopropylacrylamide
Temperature

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Yamanishi, Y., Teramoto, J., Magariyama, Y., Ishihama, A., Fukuda, T., & Arai, F. (2009). On-chip temperature control under suspended microbridge for cell immobilization and culture system. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1683-1685). Chemical and Biological Microsystems Society.

On-chip temperature control under suspended microbridge for cell immobilization and culture system. / Yamanishi, Yoko; Teramoto, Jun; Magariyama, Yukio; Ishihama, Akira; Fukuda, Toshio; Arai, Fumihito.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1683-1685.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yamanishi, Y, Teramoto, J, Magariyama, Y, Ishihama, A, Fukuda, T & Arai, F 2009, On-chip temperature control under suspended microbridge for cell immobilization and culture system. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1683-1685, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 11/1/09.
Yamanishi Y, Teramoto J, Magariyama Y, Ishihama A, Fukuda T, Arai F. On-chip temperature control under suspended microbridge for cell immobilization and culture system. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 1683-1685
Yamanishi, Yoko ; Teramoto, Jun ; Magariyama, Yukio ; Ishihama, Akira ; Fukuda, Toshio ; Arai, Fumihito. / On-chip temperature control under suspended microbridge for cell immobilization and culture system. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 1683-1685
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