Abstract
We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.
Original language | English |
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Title of host publication | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | Chemical and Biological Microsystems Society |
Pages | 1683-1685 |
Number of pages | 3 |
ISBN (Print) | 9780979806421 |
Publication status | Published - 2009 |
Externally published | Yes |
Event | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: Nov 1 2009 → Nov 5 2009 |
Other
Other | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 |
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Country/Territory | Korea, Republic of |
City | Jeju |
Period | 11/1/09 → 11/5/09 |
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering