On-chip transportation and measurement of mechanical characteristics of oocytes in an open environment

Kou Nakahara, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We propose a system that transports oocytes and measures their mechanical characteristics in an open environment using a robot integrated microfluidic chip (chip). The cells are transported through a micropillar array in the chip, and their characteristics are measured by a mechanical probe and a force sensor. Because the chip has an open microchannel, important cells such as oocytes are easily introduced and collected without the risk for losing them. In addition, any bubbles trapped in the chip, which degrade the measurement precision, are easily removed. To transport the oocytes through the open microchannel, we adopt a transportation technique based on a vibration-induced flow. Under this flow, oocytes arrive at the measurement point, where their mechanical characteristics are determined. We demonstrate the introduction, transportation, measurement of mechanical characteristics, and collection of oocytes using this system.

Original languageEnglish
Pages (from-to)648-659
Number of pages12
JournalMicromachines
Volume6
Issue number5
DOIs
Publication statusPublished - 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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