On-line redox derivatization liquid chromatography using a carbon monolithic column

Ken Miyashita, Ryo Tanaka, George Hasegawa, Kazuki Nakanishi, Kazuhiro Morioka, Hulie Zeng, Shungo Kato, Katsumi Uchiyama, Kazunori Saitoh, Masami Shibukawa, Hizuru Nakajima

Research output: Contribution to journalArticle

Abstract

A macroporous resorcinol-formaldehyde (RF) xerogel was synthesized via sol–gel reaction, accompanied by phase separation; a graphitized carbon monolith was then prepared by calcining the RF xerogel at a relatively low temperature (1200 °C) utilizing catalytic graphitization. The retention behaviors of metal-EDTA complexes on the carbon monolithic column were investigated. The carbon monolith exhibited catalytic activity for redox reactions, like porous graphitic carbon packing materials do; they oxidized Co(II)-EDTA to Co(III)-EDTA during elution. Their redox activity could be modified by treating with an eluent containing an oxidizing or reducing agent. A novel on-line redox derivatization HPLC system using a carbon monolithic column was developed for enhancing the separation selectivity. This on-line redox derivatization HPLC system consisted of two ODS columns as a separation column and one carbon monolithic column as redox derivatization unit placed between them. The Co(II)-EDTA migrated as its original oxidation state in the first ODS column, while it migrated as Co(III)-EDTA in the second ODS column, since Co(II)-EDTA was rapidly oxidized to Co(III)-EDTA in the carbon monolithic column. On the other hand, other metal-EDTA complexes migrated as their original oxidation states in these columns. Therefore, the Co-EDTA could be separated from the other metal-EDTA complexes. This on-line redox derivatization HPLC system was successfully used for determining the trace amount of cobalt in a reference copper alloy.

Original languageEnglish
Pages (from-to)469-478
Number of pages10
Journalbunseki kagaku
Volume67
Issue number8
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Liquid chromatography
Edetic Acid
Carbon
Xerogels
Metals
Formaldehyde
Oxidation-Reduction
Oxidation
Graphitization
Redox reactions
Copper alloys
Reducing Agents
Cobalt
Oxidants
Phase separation
Catalyst activity

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Miyashita, K., Tanaka, R., Hasegawa, G., Nakanishi, K., Morioka, K., Zeng, H., ... Nakajima, H. (2018). On-line redox derivatization liquid chromatography using a carbon monolithic column. bunseki kagaku, 67(8), 469-478. https://doi.org/10.2116/bunsekikagaku.67.469

On-line redox derivatization liquid chromatography using a carbon monolithic column. / Miyashita, Ken; Tanaka, Ryo; Hasegawa, George; Nakanishi, Kazuki; Morioka, Kazuhiro; Zeng, Hulie; Kato, Shungo; Uchiyama, Katsumi; Saitoh, Kazunori; Shibukawa, Masami; Nakajima, Hizuru.

In: bunseki kagaku, Vol. 67, No. 8, 01.01.2018, p. 469-478.

Research output: Contribution to journalArticle

Miyashita, K, Tanaka, R, Hasegawa, G, Nakanishi, K, Morioka, K, Zeng, H, Kato, S, Uchiyama, K, Saitoh, K, Shibukawa, M & Nakajima, H 2018, 'On-line redox derivatization liquid chromatography using a carbon monolithic column', bunseki kagaku, vol. 67, no. 8, pp. 469-478. https://doi.org/10.2116/bunsekikagaku.67.469
Miyashita K, Tanaka R, Hasegawa G, Nakanishi K, Morioka K, Zeng H et al. On-line redox derivatization liquid chromatography using a carbon monolithic column. bunseki kagaku. 2018 Jan 1;67(8):469-478. https://doi.org/10.2116/bunsekikagaku.67.469
Miyashita, Ken ; Tanaka, Ryo ; Hasegawa, George ; Nakanishi, Kazuki ; Morioka, Kazuhiro ; Zeng, Hulie ; Kato, Shungo ; Uchiyama, Katsumi ; Saitoh, Kazunori ; Shibukawa, Masami ; Nakajima, Hizuru. / On-line redox derivatization liquid chromatography using a carbon monolithic column. In: bunseki kagaku. 2018 ; Vol. 67, No. 8. pp. 469-478.
@article{ba017c3e1ab34f278674475e12916700,
title = "On-line redox derivatization liquid chromatography using a carbon monolithic column",
abstract = "A macroporous resorcinol-formaldehyde (RF) xerogel was synthesized via sol–gel reaction, accompanied by phase separation; a graphitized carbon monolith was then prepared by calcining the RF xerogel at a relatively low temperature (1200 °C) utilizing catalytic graphitization. The retention behaviors of metal-EDTA complexes on the carbon monolithic column were investigated. The carbon monolith exhibited catalytic activity for redox reactions, like porous graphitic carbon packing materials do; they oxidized Co(II)-EDTA to Co(III)-EDTA during elution. Their redox activity could be modified by treating with an eluent containing an oxidizing or reducing agent. A novel on-line redox derivatization HPLC system using a carbon monolithic column was developed for enhancing the separation selectivity. This on-line redox derivatization HPLC system consisted of two ODS columns as a separation column and one carbon monolithic column as redox derivatization unit placed between them. The Co(II)-EDTA migrated as its original oxidation state in the first ODS column, while it migrated as Co(III)-EDTA in the second ODS column, since Co(II)-EDTA was rapidly oxidized to Co(III)-EDTA in the carbon monolithic column. On the other hand, other metal-EDTA complexes migrated as their original oxidation states in these columns. Therefore, the Co-EDTA could be separated from the other metal-EDTA complexes. This on-line redox derivatization HPLC system was successfully used for determining the trace amount of cobalt in a reference copper alloy.",
author = "Ken Miyashita and Ryo Tanaka and George Hasegawa and Kazuki Nakanishi and Kazuhiro Morioka and Hulie Zeng and Shungo Kato and Katsumi Uchiyama and Kazunori Saitoh and Masami Shibukawa and Hizuru Nakajima",
year = "2018",
month = "1",
day = "1",
doi = "10.2116/bunsekikagaku.67.469",
language = "English",
volume = "67",
pages = "469--478",
journal = "Bunseki Kagaku",
issn = "0525-1931",
publisher = "公益社団法人日本分析化学会",
number = "8",

}

TY - JOUR

T1 - On-line redox derivatization liquid chromatography using a carbon monolithic column

AU - Miyashita, Ken

AU - Tanaka, Ryo

AU - Hasegawa, George

AU - Nakanishi, Kazuki

AU - Morioka, Kazuhiro

AU - Zeng, Hulie

AU - Kato, Shungo

AU - Uchiyama, Katsumi

AU - Saitoh, Kazunori

AU - Shibukawa, Masami

AU - Nakajima, Hizuru

PY - 2018/1/1

Y1 - 2018/1/1

N2 - A macroporous resorcinol-formaldehyde (RF) xerogel was synthesized via sol–gel reaction, accompanied by phase separation; a graphitized carbon monolith was then prepared by calcining the RF xerogel at a relatively low temperature (1200 °C) utilizing catalytic graphitization. The retention behaviors of metal-EDTA complexes on the carbon monolithic column were investigated. The carbon monolith exhibited catalytic activity for redox reactions, like porous graphitic carbon packing materials do; they oxidized Co(II)-EDTA to Co(III)-EDTA during elution. Their redox activity could be modified by treating with an eluent containing an oxidizing or reducing agent. A novel on-line redox derivatization HPLC system using a carbon monolithic column was developed for enhancing the separation selectivity. This on-line redox derivatization HPLC system consisted of two ODS columns as a separation column and one carbon monolithic column as redox derivatization unit placed between them. The Co(II)-EDTA migrated as its original oxidation state in the first ODS column, while it migrated as Co(III)-EDTA in the second ODS column, since Co(II)-EDTA was rapidly oxidized to Co(III)-EDTA in the carbon monolithic column. On the other hand, other metal-EDTA complexes migrated as their original oxidation states in these columns. Therefore, the Co-EDTA could be separated from the other metal-EDTA complexes. This on-line redox derivatization HPLC system was successfully used for determining the trace amount of cobalt in a reference copper alloy.

AB - A macroporous resorcinol-formaldehyde (RF) xerogel was synthesized via sol–gel reaction, accompanied by phase separation; a graphitized carbon monolith was then prepared by calcining the RF xerogel at a relatively low temperature (1200 °C) utilizing catalytic graphitization. The retention behaviors of metal-EDTA complexes on the carbon monolithic column were investigated. The carbon monolith exhibited catalytic activity for redox reactions, like porous graphitic carbon packing materials do; they oxidized Co(II)-EDTA to Co(III)-EDTA during elution. Their redox activity could be modified by treating with an eluent containing an oxidizing or reducing agent. A novel on-line redox derivatization HPLC system using a carbon monolithic column was developed for enhancing the separation selectivity. This on-line redox derivatization HPLC system consisted of two ODS columns as a separation column and one carbon monolithic column as redox derivatization unit placed between them. The Co(II)-EDTA migrated as its original oxidation state in the first ODS column, while it migrated as Co(III)-EDTA in the second ODS column, since Co(II)-EDTA was rapidly oxidized to Co(III)-EDTA in the carbon monolithic column. On the other hand, other metal-EDTA complexes migrated as their original oxidation states in these columns. Therefore, the Co-EDTA could be separated from the other metal-EDTA complexes. This on-line redox derivatization HPLC system was successfully used for determining the trace amount of cobalt in a reference copper alloy.

UR - http://www.scopus.com/inward/record.url?scp=85053424956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053424956&partnerID=8YFLogxK

U2 - 10.2116/bunsekikagaku.67.469

DO - 10.2116/bunsekikagaku.67.469

M3 - Article

AN - SCOPUS:85053424956

VL - 67

SP - 469

EP - 478

JO - Bunseki Kagaku

JF - Bunseki Kagaku

SN - 0525-1931

IS - 8

ER -