One-dimensional assembly of functional proteins: Toward the design of an artificial cellulosome

Y. Mori, H. Nakazawa, G. A.L. Gonçalves, T. Tanaka, M. Umetsu, Noriho Kamiya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In biological systems, proteins can form well-organized, higher-order structures with unique functions that would be difficult to achieve with a single protein. These proteinaceous supramolecular structures form by self-assembly, and the spatial arrangement of the protein building blocks in them is very important. In the present study, an artificial system was developed using recombinant proteins as building blocks, which were assembled in a one-dimensional manner. The assembly of these building blocks was based on the avidin-biotin interaction. A tetrameric biotin ligand unit was designed so that the 1:4 stoichiometry of the avidin-biotin interaction was altered to a 1:2 directional interaction between the streptavidin and tetrabiotinylated protein units. In a proof-of-concept study, site-specifically tetrabiotin-labeled endoglucanase and cellulose-binding module units were prepared, and then these components were self-assembled by mixing with streptavidin to mimic a natural cellulosome. The formation of one-dimensional assemblies of the protein units depended on the stoichiometry of the avidin-biotin interaction sites in the system. Interestingly, the saccharification efficiency improved when the component ratio of protein units in the assemblies was changed. The presence of the optimal ratio of the building blocks implies the modularity of the present protein assembly system.

Original languageEnglish
Pages (from-to)66-73
Number of pages8
JournalMolecular Systems Design and Engineering
Volume1
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Proteins
Biotin
Avidin
Streptavidin
Stoichiometry
Saccharification
Recombinant proteins
Cellulase
Biological systems
Recombinant Proteins
Cellulose
Self assembly
Ligands

All Science Journal Classification (ASJC) codes

  • Chemistry (miscellaneous)
  • Chemical Engineering (miscellaneous)
  • Biomedical Engineering
  • Energy Engineering and Power Technology
  • Process Chemistry and Technology
  • Industrial and Manufacturing Engineering
  • Materials Chemistry

Cite this

One-dimensional assembly of functional proteins : Toward the design of an artificial cellulosome. / Mori, Y.; Nakazawa, H.; Gonçalves, G. A.L.; Tanaka, T.; Umetsu, M.; Kamiya, Noriho.

In: Molecular Systems Design and Engineering, Vol. 1, No. 1, 01.01.2016, p. 66-73.

Research output: Contribution to journalArticle

Mori, Y. ; Nakazawa, H. ; Gonçalves, G. A.L. ; Tanaka, T. ; Umetsu, M. ; Kamiya, Noriho. / One-dimensional assembly of functional proteins : Toward the design of an artificial cellulosome. In: Molecular Systems Design and Engineering. 2016 ; Vol. 1, No. 1. pp. 66-73.
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